Molecular mechanisms underlying natural deficient and ultraviolet-induced accumulation of anthocyanin in the peel of 'Jinxiu' peach.

Autor: Liang L; College of Agriculture & Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou, China., Zhu J; New Zealand Institute for Plant & Food Research Limited, Auckland, New Zealand.; School of Biological Sciences, University of Auckland, Auckland, New Zealand., Huang D; College of Agriculture & Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou, China., Ai S; Zhejiang Provincial Key Laboratory of Horticultural Crop Quality Manipulation, Zijingang Campus, Zhejiang University, Hangzhou, China., Xue L; College of Agriculture & Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou, China., Yin X; School of Horticulture, Anhui Agricultural University, Hefei, China., Lin-Wang K; New Zealand Institute for Plant & Food Research Limited, Auckland, New Zealand., Allan A; New Zealand Institute for Plant & Food Research Limited, Auckland, New Zealand.; School of Biological Sciences, University of Auckland, Auckland, New Zealand., Chen K; College of Agriculture & Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou, China.; Zhejiang Provincial Key Laboratory of Horticultural Crop Quality Manipulation, Zijingang Campus, Zhejiang University, Hangzhou, China.; The State Agriculture Ministry Laboratory of Horticultural Plant Crop Growth and Development, Zijingang Campus, Zhejiang University, Hangzhou, China., Xu C; College of Agriculture & Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou, China.; Zhejiang Provincial Key Laboratory of Horticultural Crop Quality Manipulation, Zijingang Campus, Zhejiang University, Hangzhou, China.; The State Agriculture Ministry Laboratory of Horticultural Plant Crop Growth and Development, Zijingang Campus, Zhejiang University, Hangzhou, China.
Jazyk: angličtina
Zdroj: Plant, cell & environment [Plant Cell Environ] 2024 Dec; Vol. 47 (12), pp. 4833-4848. Date of Electronic Publication: 2024 Aug 05.
DOI: 10.1111/pce.15064
Abstrakt: Peach varieties that differ in red coloration due to varied anthocyanin accumulation result from transcriptional regulation by PpMYB10s, a group of specific R2R3 MYBs. Here we investigated the mechanisms driving a lack of anthocyanin in yellow-skinned 'Jinxiu' peach peel, as well as accumulation induced by UV irradiance. It was found that PpMYB10.1, PpMYB10.2 and PpMYB10.3 were positive regulators of anthocyanin accumulation, but the stimulation by PpMYB10.2 was weak. Low expression of PpMYB10.1 causes natural anthocyanin deficiency in 'Jinxiu' peel. However, the promoter sequences of PpMYB10.1 were identical in 'Jinxiu' and a naturally red-coloured peach 'Hujingmilu'. Therefore, potential negative regulator(s) upstream of PpMYB10.1 were explored. A novel R2R3-MYB repressor termed PpMYB80 was identified through comparative transcriptomic analysis and then functionally confirmed via transiently overexpressing and silencing in peach fruit, as well as transformation in tobacco. PpMYB80 directly binds to the promoter of PpMYB10.1 and inhibits its expression, but does not affect PpMYB10.3. In UV-exposed 'Jinxiu' fruit, expression of PpMYB10.3 was upregulated, while PpMYB10.1 remained low and PpMYB80 enhanced, which results in accumulation of anthocyanin in peel. This study revealed a transcriptional cascade involving PpMYB activators and repressors in regulating basal and UV-induced anthocyanin accumulation in peach peel.
(© 2024 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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