Decreased expression of p53 is associated with down expression of zyxin in breast cancer.

Autor: Rostampour R; Department of Clinical Biochemistry Kermanshah University of Medical Sciences Kermanshah Iran., Bahremand K; Nano Drug Delivery Research Center, Health Technology Institute Kermanshah University of Medical Sciences Kermanshah Iran., Mohammadi H; Department of Clinical Biochemistry Kermanshah University of Medical Sciences Kermanshah Iran., Roghani SA; Clinical Research Development Center, Imam Reza Hospital Kermanshah University of Medical Science Kermanshah Iran., Shakiba E; Department of Clinical Biochemistry Kermanshah University of Medical Sciences Kermanshah Iran., Goodarzi MT; Department of Biochemistry Shahrood Branch, Islamic Azad University Shahrood Iran., Asadi S; Department of Clinical Biochemistry Kermanshah University of Medical Sciences Kermanshah Iran.
Jazyk: angličtina
Zdroj: Health science reports [Health Sci Rep] 2024 Aug 01; Vol. 7 (8), pp. e2288. Date of Electronic Publication: 2024 Aug 01 (Print Publication: 2024).
DOI: 10.1002/hsr2.2288
Abstrakt: Background and Aims: Breast cancer (BC) is considered one of the most common malignant tumors leading to death in women, and genetic factors have a crucial role in BC pathogenesis. Zyxin (ZYX) is one of these factors that may be important in p53 level and function. Thus, the present work aimed to investigate the ZYX gene and protein expression in tumor tissue and matched margin tissue and its correlation with the p53 expression.
Methods: In a present case-control study, 30 tumors and 30 matched margin tissues were obtained from Iran Tumor Bank/Tehran University of Medical Sciences. Real-time polymerase chain reaction and western blot analysis techniques were applied to evaluate the genes and protein expression, respectively.
Results: The data showed that expression of the ZYX gene in tumor tissues significantly decreased ( p  = 0.0274) compared to matched margin tissues. In contrast, the p53 gene expression in tumor tissues had no significant difference with matched margin tissues. Additionally, we observed that ZYX and p53 genes expression in tumor tissues of estrogen receptor-positive patients had significant elevation than estrogen receptor-negative patients ( p  < 0.001, p  < 0.001, respectively). The data of the western blot analysis technique showed that protein expression of ZYX ( p  = 0.0024) and P53 protein ( p  = 0.0218) in tumor tissues was significantly reduced compared to matched margin tissues. Additionally, our analysis showed a direct and significant correlation between the expression of ZYX and p53 proteins ( r  = 0.7797, p  = 0.0126) and expression of ZYX and p53 genes ( r  = 0.3079, p  = 0.0187).
Conclusion: Based on our observation, ZYX might have a tumor suppressor role and is associated with p53.
Competing Interests: The authors declare no conflict of interest.
(© 2024 The Author(s). Health Science Reports published by Wiley Periodicals LLC.)
Databáze: MEDLINE
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