Well Plate-Based Localized Electroporation Workflow for Rapid Optimization of Intracellular Delivery.

Autor:	Patino CA; Department of Mechanical Engineering, Northwestern University, Evanston, IL, USA., Sarikaya S; Department of Mechanical Engineering, Northwestern University, Evanston, IL, USA., Mukherjee P; Department of Mechanical Engineering, Northwestern University, Evanston, IL, USA.; Theoretical and Applied Mechanics Program, Northwestern University, Evanston, IL, USA., Pathak N; Department of Mechanical Engineering, Northwestern University, Evanston, IL, USA.; Theoretical and Applied Mechanics Program, Northwestern University, Evanston, IL, USA., Espinosa HD; Department of Mechanical Engineering, Northwestern University, Evanston, IL, USA.; Theoretical and Applied Mechanics Program, Northwestern University, Evanston, IL, USA.
Jazyk:	angličtina
Zdroj:	Bio-protocol [Bio Protoc] 2024 Jul 20; Vol. 14 (14), pp. e5037. Date of Electronic Publication: 2024 Jul 20 (Print Publication: 2024).
DOI:	10.21769/BioProtoc.5037
Abstrakt:	Efficient and nontoxic delivery of foreign cargo into cells is a critical step in many biological studies and cell engineering workflows with applications in areas such as biomanufacturing and cell-based therapeutics. However, effective molecular delivery into cells involves optimizing several experimental parameters. In the case of electroporation-based intracellular delivery, there is a need to optimize parameters like pulse voltage, duration, buffer type, and cargo concentration for each unique application. Here, we present the protocol for fabricating and utilizing a high-throughput multi-well localized electroporation device (LEPD) assisted by deep learning-based image analysis to enable rapid optimization of experimental parameters for efficient and nontoxic molecular delivery into cells. The LEPD and the optimization workflow presented herein are relevant to both adherent and suspended cell types and different molecular cargo (DNA, RNA, and proteins). The workflow enables multiplexed combinatorial experiments and can be adapted to cell engineering applications requiring in vitro delivery. Key features • A high-throughput multi-well localized electroporation device (LEPD) that can be optimized for both adherent and suspended cell types. • Allows for multiplexed experiments combined with tailored pulse voltage, duration, buffer type, and cargo concentration. • Compatible with various molecular cargoes, including DNA, RNA, and proteins, enhancing its versatility for cell engineering applications. • Integration with deep learning-based image analysis enables rapid optimization of experimental parameters. Competing Interests: Competing interestsThe authors declare that they have no competing interests. (©Copyright : © 2024 The Authors; This is an open access article under the CC BY license.)
Databáze:	MEDLINE
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