Dual-Target Additively Manufactured Electrochemical Sensor for the Multiplexed Detection of Protein A29 and DNA of Human Monkeypox Virus.
| Autor: | Silva LRG; Laboratory of Sensors, Nanomedicine and Nanostructured Materials, Federal University of São Carlos, Araras 13600-970, Brazil., Stefano JS; Laboratory of Sensors, Nanomedicine and Nanostructured Materials, Federal University of São Carlos, Araras 13600-970, Brazil., Kalinke C; Institute of Chemistry, University of Campinas (Unicamp), São Paulo 13083-859, Brazil., Crapnell RD; Faculty of Science and Engineering, Manchester Metropolitan University, Chester Street, Manchester M1 5GD, United Kingdom., Brazaca LC; São Carlos Institute of Chemistry, University of São Paulo, São Carlos, SP 13083-970, Brazil., Marcolino-Junior LH; Chemistry Department, Laboratory of Electrochemical Sensors (LabSensE), Federal University of Paraná, Curitiba, PR 81531-980, Brazil., Bergamini MF; Chemistry Department, Laboratory of Electrochemical Sensors (LabSensE), Federal University of Paraná, Curitiba, PR 81531-980, Brazil., Banks CE; Faculty of Science and Engineering, Manchester Metropolitan University, Chester Street, Manchester M1 5GD, United Kingdom., Janegitz BC; Laboratory of Sensors, Nanomedicine and Nanostructured Materials, Federal University of São Carlos, Araras 13600-970, Brazil. |
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| Jazyk: | angličtina |
| Zdroj: | ACS omega [ACS Omega] 2024 Jul 17; Vol. 9 (30), pp. 33099-33110. Date of Electronic Publication: 2024 Jul 17 (Print Publication: 2024). |
| DOI: | 10.1021/acsomega.4c04460 |
| Abstrakt: | Herein, we present the first 3D-printed electrochemical portable biodevice for the detection of monkeypox virus (MKPV). The electrochemical device consists of two biosensors: an immunosensor and a genosensor specifically designed for the detection of the protein A29 and a target DNA of MKPV, respectively. The electrodes were manufactured using lab-made ultraflexible conductive filaments composed of carbon black, recycled PLA from coffee pods, and castor oil as a plasticizer. The sensors created through 3D printing technology exhibited good reproducibility and repeatability of analytical responses. Furthermore, both the immunosensor and genosensor demonstrated excellent MKPV detection capabilities, with a linear range from 0.01 to 1.0 μmol L -1 for the antigen and 0.1 to 20.0 μmol L -1 for the DNA target. The biosensors achieved limits of detection of 2.7 and 29 nmol L -1 for the immunosensor and genosensor, respectively. Interference tests conducted with the biosensors demonstrated their selectivity for MKPV. Moreover, analyses of fortified human serum samples showed recoveries close to 100%, confirming the absence of significant matrix effects for MKPV analysis. Therefore, the 3D-printed multiplex device represents a viable and highly promising alternative for on-site, portable, and rapid point-of-care MKPV monitoring. Competing Interests: The authors declare no competing financial interest. (© 2024 The Authors. Published by American Chemical Society.) |
| Databáze: | MEDLINE |
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