Melatonin targets the paraventricular thalamus to promote non-rapid eye movement sleep in C3H/HeJ mice.

Autor:	Wang Y; Department of Physiology, College of Basic Medical Sciences, Army Medical University, Chongqing 400038, China. Electronic address: anggelly@163.com., Song Z; Department of Physiology, College of Basic Medical Sciences, Army Medical University, Chongqing 400038, China., Han Q; Department of Radiology, Southwest Hospital, Army Medical University, Chongqing 400038, China., Luo F; Department of Physiology, College of Basic Medical Sciences, Army Medical University, Chongqing 400038, China., Jiang C; Department of Medical Psychology, Chongqing Health Center for Women and Children, Chongqing 401147, China., Zhang Z; Department of Physiology, College of Basic Medical Sciences, Jilin University, Changchun 130021, China., Wang N; College of Bioengineering, Chongqing University, Chongqing 400044, China., Zou N; Chongqing Institute for Brain and Intelligence, Guangyang Bay Laboratory, Chongqing 400064, China., Liu G; Chongqing Institute for Brain and Intelligence, Guangyang Bay Laboratory, Chongqing 400064, China., Long M; Department of Physiology, College of Basic Medical Sciences, Army Medical University, Chongqing 400038, China., Liu H; Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China., Xiao Q; Department of Physiology, College of Basic Medical Sciences, Army Medical University, Chongqing 400038, China., Yue F; Sleep and Psychology Center, Bishan Hospital of Chongqing Medical University, Chongqing 402760, China., Xia J; Department of Physiology, College of Basic Medical Sciences, Army Medical University, Chongqing 400038, China., He C; Department of Physiology, College of Basic Medical Sciences, Army Medical University, Chongqing 400038, China., Hu Z; Department of Physiology, College of Basic Medical Sciences, Army Medical University, Chongqing 400038, China; Chongqing Institute for Brain and Intelligence, Guangyang Bay Laboratory, Chongqing 400064, China. Electronic address: zhianhu@aliyun.com., Ren S; Department of Physiology, College of Basic Medical Sciences, Army Medical University, Chongqing 400038, China. Electronic address: shuan0808@163.com.
Jazyk:	angličtina
Zdroj:	Current biology : CB [Curr Biol] 2024 Aug 19; Vol. 34 (16), pp. 3792-3803.e5. Date of Electronic Publication: 2024 Aug 02.
DOI:	10.1016/j.cub.2024.07.033
Abstrakt:	Melatonin (MLT) is an important circadian signal for sleep regulation, but the neural circuitries underlying the sleep-promoting effects of MLT are poorly understood. The paraventricular thalamus (PVT) is a critical thalamic area for wakefulness control and expresses MLT receptors, raising a possibility that PVT neurons may mediate the sleep-promoting effects of MLT. Here, we found that MLT receptors were densely expressed on PVT neurons and exhibited circadian-dependent variations in C3H/HeJ mice. Application of exogenous MLT decreased the excitability of PVT neurons, resulting in hyperpolarization of membrane potential and reduction of action potential firing. MLT also inhibited the spontaneous activity of PVT neurons at both population and single-neuron levels in freely behaving mice. Furthermore, pharmacological manipulations revealed that local infusion of exogeneous MLT into the PVT promoted non-rapid eye movement (NREM) sleep and increased NREM sleep duration, whereas MLT receptor antagonists decreased NREM sleep. Moreover, we found that selectively knocking down endogenous MLT receptors in the PVT decreased NREM sleep and correspondingly increased wakefulness, with particular changes shortly after the onset of the dark or light phase. Taken together, these results demonstrate that PVT is an important target of MLT for promoting NREM sleep. Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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