The biological characteristics of DAstV molecular epidemiology and pathogenicity of duck astrovirus causing hepatitis in ducks and chickens in Southeast China.

Autor:	Xu J; College of Animal Science, South China Agricultural University, Guangzhou 510642, China., Yin L; Guangdong Wens Food Group, Yunfu 527499, China., Guo Y; College of Animal Science, South China Agricultural University, Guangzhou 510642, China., Yan Z; Guangdong Wens Food Group, Yunfu 527499, China., Yu S; Guangdong Wens Food Group, Yunfu 527499, China., Jiang T; Guangdong Wens Food Group, Yunfu 527499, China., Liao X; Guangdong Wens Food Group, Yunfu 527499, China., Lin W; College of Animal Science, South China Agricultural University, Guangzhou 510642, China., Chen F; College of Animal Science, South China Agricultural University, Guangzhou 510642, China. Electronic address: fengch@scau.edu.cn.
Jazyk:	angličtina
Zdroj:	Poultry science [Poult Sci] 2024 Oct; Vol. 103 (10), pp. 104076. Date of Electronic Publication: 2024 Jul 10.
DOI:	10.1016/j.psj.2024.104076
Abstrakt:	A suspected outbreak of duck astrovirus (DAstV) disease occurred in a large Muscovy duck farm in Guangdong Province, China, in June 2022, which severely affected the production performance and health of Muscovy ducks. This study aimed to investigate the prevalence of DAstV disease in Southeast China. Herein, we employed semi-nested PCR ethodto screen 5203 swab and liver samples from 11 Muscovy duck farms in 5 provinces of China for the presence of DAstV. Among them, 1356 samples (26.06%, 1356/5203) tested positive for DAstV, out of which 11 DAstV strains were isolated after 10 generations of blind transmission through Leghorn male hepatoma (LMH) cells and performed their whole-genome sequencing. The alignment results showed that the 11 DAstV isolates exhibited relatively low homology (15.4%-75%) with the astrovirus isolates from other species published in GenBank, whereas their homology (nucleotide: 90.4%-99.99%; amino acid: 94%-99.8%) with the DAstV type 1 (DAstV-1) reference strain was higher, indicating considerable homology. The results indicated that DAstV-1 was the main pathogenic factor. Herein, we successfully recreated the clinical symptoms of natural infection in 28-day-old specific-pathogen-free (SPF) ducks using the DAstV-1-GDB-2022 strain. The primary clinical manifestations included liver enlargement, hemorrhaging, and disruptions in liver function. Additionally, we confirmed the cross-species transmission potential of DAstV-1, marking the first occurrence of clinical symptoms of DAstV in 28-day-old SPF chickens. Our findings provide new perspectives on the epidemiology and pathogenicity of DAstV-1 and may help in advancing the development of DAstV vaccines. Competing Interests: DISCLOSURES No conflict of interest exits in the submission of this manuscript. All the authors listed have approved the manuscript that is enclosed. (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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