Cytokinin oxidase2-deficient mutants improve panicle and grain architecture through cytokinin accumulation and enhance drought tolerance in indica rice.

Autor: Rashid A; Crop Improvement Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, Delhi, India, 110067.; Department of Biotechnology, Centre for Transgenic Plant Development, Jamia Hamdard, New Delhi, India, 110062., Achary VMM; Crop Improvement Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, Delhi, India, 110067. vmmachary@gmail.com., Abdin MZ; Department of Biotechnology, Centre for Transgenic Plant Development, Jamia Hamdard, New Delhi, India, 110062., Karippadakam S; Crop Improvement Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, Delhi, India, 110067., Parmar H; Crop Improvement Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, Delhi, India, 110067., Panditi V; Crop Improvement Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, Delhi, India, 110067., Prakash G; Plant Pathology, ICAR-Indian Agricultural Research Institute (IARI), New Delhi, India, 110012., Bhatnagar-Mathur P; Plant Breeding and Genetics, International Atomic Energy Agency (IAEA), PO-1001400, Vienna, Austria., Reddy MK; Crop Improvement Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, Delhi, India, 110067.
Jazyk: angličtina
Zdroj: Plant cell reports [Plant Cell Rep] 2024 Aug 03; Vol. 43 (8), pp. 207. Date of Electronic Publication: 2024 Aug 03.
DOI: 10.1007/s00299-024-03289-6
Abstrakt: Key Message: The Osckx2 mutant accumulates cytokinin thereby enhancing panicle branching, grain yield, and drought tolerance, marked by improved survival rate, membrane integrity, and photosynthetic function. Cytokinins (CKs) are multifaceted hormones that regulate growth, development, and stress responses in plants. Cytokinins have been implicated in improved panicle architecture and grain yield; however, they are inactivated by the enzyme cytokinin oxidase (CKX). In this study, we developed a cytokinin oxidase 2 (Osckx2)-deficient mutant using CRISPR/Cas9 gene editing in indica rice and assessed its function under water-deficit and salinity conditions. Loss of OsCKX2 function increased grain number, secondary panicle branching, and overall grain yield through improved cytokinin content in the panicle tissue. Under drought conditions, the Osckx2 mutant conserved more water and demonstrated improved water-saving traits. Through reduced transpiration, Osckx2 mutants showed an improved survival response than the wild type to unset dehydration stress. Further, Osckx2 maintained chloroplast and membrane integrity and showed significantly improved photosynthetic function under drought conditions through enhanced antioxidant protection systems. The OsCKX2 function negatively affects panicle grain number and drought tolerance, with no discernible impact in response to salinity. The finding suggests the utility of the beneficial Osckx2 allele in breeding to develop climate-resilient, high-yielding cultivars for future food security.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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