Allelic variability in the Rpp1 locus conferring resistance to Asian soybean rust revealed by genome-wide association.

Autor: Aoyagi LN; National Agriculture and Food Research Organization (NARO), 3-1-3 Kannondai, Tsukuba, Ibaraki, 305-8604, Japan.; Maringá State University (UEM), Colombo Avenue, No. 5790, Maringá, PR, Brazil., Ferreira EGC; 2Blades, Evanston, IL, USA.; The Sainsbury Laboratory, Norwich, UK., da Silva DCG; Brazilian Agricultural Research Corporation - National Soybean Research Center (Embrapa Soja), Carlos João Strass Road, Warta County, Londrina, PR, Brazil., Dos Santos AB; Brazilian Agricultural Research Corporation - National Soybean Research Center (Embrapa Soja), Carlos João Strass Road, Warta County, Londrina, PR, Brazil., Avelino BB; Department of Computer Science, Federal University of Technology of Paraná (UTFPR), Paraná, Brazil., Lopes-Caitar VS; Department of Plant Sciences, University of Tennessee, Knoxville, TN, USA., de Oliveira MF; Brazilian Agricultural Research Corporation - National Soybean Research Center (Embrapa Soja), Carlos João Strass Road, Warta County, Londrina, PR, Brazil., Abdelnoor RV; Brazilian Agricultural Research Corporation - National Soybean Research Center (Embrapa Soja), Carlos João Strass Road, Warta County, Londrina, PR, Brazil., de Souto ER; Maringá State University (UEM), Colombo Avenue, No. 5790, Maringá, PR, Brazil., Arias CA; Brazilian Agricultural Research Corporation - National Soybean Research Center (Embrapa Soja), Carlos João Strass Road, Warta County, Londrina, PR, Brazil., Belzile F; Department of Plant Sciences and Institute of Integrative Biology and Systems (IBIS), Université Laval, Quebec City, Quebec, G1V 0A6, Canada., Marcelino-Guimarães FC; Brazilian Agricultural Research Corporation - National Soybean Research Center (Embrapa Soja), Carlos João Strass Road, Warta County, Londrina, PR, Brazil. francismar.marcelino@embrapa.br.
Jazyk: angličtina
Zdroj: BMC plant biology [BMC Plant Biol] 2024 Aug 03; Vol. 24 (1), pp. 743. Date of Electronic Publication: 2024 Aug 03.
DOI: 10.1186/s12870-024-05454-1
Abstrakt: Soybean is a crucial crop for the Brazilian economy, but it faces challenges from the biotrophic fungus Phakopsora pachyrhizi, which causes Asian Soybean Rust (ASR). In this study, we aimed to identify SNPs associated with resistance within the Rpp1 locus, which is effective against Brazilian ASR populations. We employed GWAS and re-sequencing analyzes to pinpoint SNP markers capable of differentiating between soybean accessions harboring the Rpp1, Rpp1-b and other alternative alleles in the Rpp1 locus and from susceptible soybean cultivars. Seven SNP markers were found to be associated with ASR resistance through GWAS, with three of them defining haplotypes that efficiently distinguished the accessions based on their ASR resistance and source of the Rpp gene. These haplotypes were subsequently validated using a bi-parental population and a diverse set of Rpp sources, demonstrating that the GWAS markers co-segregate with ASR resistance. We then examined the presence of these haplotypes in a diverse set of soybean genomes worldwide, finding a few new potential sources of Rpp1/Rpp1-b. Further genomic sequence analysis revealed nucleotide differences within the genes present in the Rpp1 locus, including the ULP1-NBS-LRR genes, which are potential R gene candidates. These results provide valuable insights into ASR resistance in soybean, thus helping the development of resistant soybean varieties through genetic breeding programs.
(© 2024. The Author(s).)
Databáze: MEDLINE
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