The fungal diversity in the lungs of children with cystic fibrosis captured by sputum-induction and bronchoalveolar lavage.

Autor: Weiser R; Microbiomes, Microbes and Informatics Group, Organisms and Environment Division, School of Biosciences, Cardiff University, Sir Martin Evans Building, Park Place, Cardiff, UK., Ronchetti K; Department of Paediatric Respiratory Medicine, Noah's Ark Children's Hospital for Wales, Cardiff, UK; Department of Paediatric Physiotherapy, Noah's Ark Children's Hospital for Wales, Cardiff, UK., Tame JD; Department of Paediatric Respiratory Medicine, Noah's Ark Children's Hospital for Wales, Cardiff, UK; Department of Paediatric Physiotherapy, Noah's Ark Children's Hospital for Wales, Cardiff, UK; School of Healthcare Sciences, Cardiff University, UK., Hoehn S; Molecular Biosciences Division, Cardiff School of Biosciences, Cardiff University, Sir Martin Evans Building, Park Place, Cardiff, UK., Jurkowski TP; Molecular Biosciences Division, Cardiff School of Biosciences, Cardiff University, Sir Martin Evans Building, Park Place, Cardiff, UK., Mahenthiralingam E; Microbiomes, Microbes and Informatics Group, Organisms and Environment Division, School of Biosciences, Cardiff University, Sir Martin Evans Building, Park Place, Cardiff, UK., Forton JT; Department of Paediatric Respiratory Medicine, Noah's Ark Children's Hospital for Wales, Cardiff, UK; School of Medicine, Cardiff University, UK. Electronic address: fortonjt@cardiff.ac.uk.
Jazyk: angličtina
Zdroj: Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society [J Cyst Fibros] 2024 Aug 01. Date of Electronic Publication: 2024 Aug 01.
DOI: 10.1016/j.jcf.2024.07.011
Abstrakt: Background: The prevalence of fungi in cystic fibrosis (CF) lung infections is poorly understood and studies have focused on adult patients. We investigated the fungal diversity in children with CF using bronchoalveolar lavage (BAL) and induced sputum (IS) samples to capture multiple lung niches.
Methods: Sequencing of the fungal ITS2 region and molecular mycobiota diversity analysis was performed on 25 matched sets of BAL-IS samples from 23 children collected as part of the CF-SpIT study (UKCRN14615; ISRCTNR12473810).
Results: Aspergillus and Candida were detected in all samples and were the most abundant and prevalent genera, followed by Dipodascus, Lecanicillium and Simplicillium. The presumptive CF pathogens Exophiala, Lomentospora and Scedosporium were identified at variable abundances in 100 %, 64 %, and 24 % of sample sets, respectively. Fungal pathogens observed at high relative abundance (≥40 %) were not accurately diagnosed by routine culture microbiology in over 50 % of the cohort. The fungal communities captured by BAL and IS samples were similar in diversity and composition, with exception to C. albicans being significantly increased in IS samples. The respiratory mycobiota varied greatly between individuals, with only 13 of 25 sample sets containing a dominant fungal taxon. In 11/25 BAL sample sets, airway compartmentalisation was observed with diverse mycobiota detected from different lobes of the lung.
Conclusions: The paediatric mycobiota is diverse, complex and inadequately diagnosed by conventional microbiology. Overlapping fungal communities were identified in BAL and IS samples, showing that IS can capture fungal genera associated with the lower airway. Compartmentalisation of the lower airway presents difficulties for consistent mycobiota sampling.
Competing Interests: Declaration of competing interest The authors do not have any conflicts of interest to disclose in relation to this study.
(Copyright © 2024. Published by Elsevier B.V.)
Databáze: MEDLINE
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