Bio-based production of cis,cis-muconic acid as platform for a sustainable polymers production.

Autor: Molinari F; Department of Biotechnology and Life Sciences, University of Insubria, via J. H. Dunant 3, 21100 Varese, Italy., Salini A; Biochemistry and Industrial Biotechnology (BIB) Laboratory, Department of Biotechnology, University of Verona, Strada le Grazie 15, 37134 Verona, Italy., Vittore A; Department of Biotechnology and Life Sciences, University of Insubria, via J. H. Dunant 3, 21100 Varese, Italy., Santoro O; Department of Biotechnology and Life Sciences, University of Insubria, via J. H. Dunant 3, 21100 Varese, Italy., Izzo L; Department of Biotechnology and Life Sciences, University of Insubria, via J. H. Dunant 3, 21100 Varese, Italy., Fusco S; Biochemistry and Industrial Biotechnology (BIB) Laboratory, Department of Biotechnology, University of Verona, Strada le Grazie 15, 37134 Verona, Italy., Pollegioni L; Department of Biotechnology and Life Sciences, University of Insubria, via J. H. Dunant 3, 21100 Varese, Italy., Rosini E; Department of Biotechnology and Life Sciences, University of Insubria, via J. H. Dunant 3, 21100 Varese, Italy. Electronic address: elena.rosini@uninsubria.it.
Jazyk: angličtina
Zdroj: Bioresource technology [Bioresour Technol] 2024 Sep; Vol. 408, pp. 131190. Date of Electronic Publication: 2024 Jul 31.
DOI: 10.1016/j.biortech.2024.131190
Abstrakt: Production of the high industrial value cis,cis-muconic acid (ccMA) from renewable biomasses is of main interest especially when biological (green) processes are used. We recently generated a E. coli strain expressing five recombinant enzymes to convert vanillin (VA, from lignin) into ccMA. Here, we optimized a growing cell approach in bioreactor for the ccMA production. The medium composition, fermentation conditions, and VA addition were tuned: pulse-feeding VA at 1 mmol/h allowed to reach 5.2 g/L of ccMA in 48 h (0.86 g ccMA/g VA), with a productivity 4-fold higher compared to the resting cells approach, thus resulting in significantly lower E-factor and Process Mass Intensity green metric parameters. The recovered ccMA has been used as building block to produce a fully bioderived polymer with rubber-like properties. The sustainable optimized bioprocess can be considered an integrated approach to develop a platform for bio-based polymers production from renewable feedstocks.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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