Activation of the helper NRC4 immune receptor forms a hexameric resistosome.

Autor:	Liu F; Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA, USA; Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA, USA., Yang Z; California Institute for Quantitative Biosciences (QB3), University of California, Berkeley, Berkeley, CA, USA; Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA, USA. Electronic address: zhenlinyang@berkeley.edu., Wang C; Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA, USA., You Z; Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA, USA., Martin R; Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA, USA., Qiao W; Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA, USA., Huang J; Department of Molecular Biology, Princeton University, Princeton, NJ, USA., Jacob P; Department of Biology and Howard Hughes Medical Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA., Dangl JL; Department of Biology and Howard Hughes Medical Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA., Carette JE; Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA, USA., Luan S; Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA, USA., Nogales E; California Institute for Quantitative Biosciences (QB3), University of California, Berkeley, Berkeley, CA, USA; Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA, USA; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA; Molecular Biophysics and Integrative Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA., Staskawicz BJ; Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA, USA; Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA, USA. Electronic address: stask@berkeley.edu.
Jazyk:	angličtina
Zdroj:	Cell [Cell] 2024 Sep 05; Vol. 187 (18), pp. 4877-4889.e15. Date of Electronic Publication: 2024 Aug 01.
DOI:	10.1016/j.cell.2024.07.013
Abstrakt:	Innate immune responses to microbial pathogens are regulated by intracellular receptors known as nucleotide-binding leucine-rich repeat receptors (NLRs) in both the plant and animal kingdoms. Across plant innate immune systems, "helper" NLRs (hNLRs) work in coordination with "sensor" NLRs (sNLRs) to modulate disease resistance signaling pathways. Activation mechanisms of hNLRs based on structures are unknown. Our research reveals that the hNLR, known as NLR required for cell death 4 (NRC4), assembles into a hexameric resistosome upon activation by the sNLR Bs2 and the pathogenic effector AvrBs2. This conformational change triggers immune responses by facilitating the influx of calcium ions (Ca 2+ ) into the cytosol. The activation mimic alleles of NRC2, NRC3, or NRC4 alone did not induce Ca 2+ influx and cell death in animal cells, suggesting that unknown plant-specific factors regulate NRCs' activation in plants. These findings significantly advance our understanding of the regulatory mechanisms governing plant immune responses. Competing Interests: Declaration of interests B.J.S. is a scientific co-founder of and serves on the board of directors for Mendel Biotechnology. He also serves on the scientific advisory boards of Verinomics and the Sainsbury Laboratory. (Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu