Exploiting CO 2 laser to boost graphite inks electron transfer for fructose biosensing in biological fluids.

Autor: Silveri F; Department of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Campus 'Aurelio Saliceti' Via R. Balzarini 1, 64100, Teramo, Italy., Della Pelle F; Department of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Campus 'Aurelio Saliceti' Via R. Balzarini 1, 64100, Teramo, Italy. Electronic address: fdellapelle@unite.it., Scroccarello A; Department of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Campus 'Aurelio Saliceti' Via R. Balzarini 1, 64100, Teramo, Italy., Bollella P; Department of Chemistry, University of Bari Aldo Moro, Via E. Orabona 4, 70125, Bari, Italy., Ferraro G; Department of Chemistry 'Ugo Schiff' and CSGI, University of Florence, Via Della Lastruccia 3, Sesto Fiorentino, 50019, Florence, Italy., Fukawa E; Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto, 606-8502, Japan., Suzuki Y; Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto, 606-8502, Japan., Sowa K; Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto, 606-8502, Japan., Torsi L; Department of Chemistry, University of Bari Aldo Moro, Via E. Orabona 4, 70125, Bari, Italy., Compagnone D; Department of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Campus 'Aurelio Saliceti' Via R. Balzarini 1, 64100, Teramo, Italy. Electronic address: dcompagnone@unite.it.
Jazyk: angličtina
Zdroj: Biosensors & bioelectronics [Biosens Bioelectron] 2024 Nov 01; Vol. 263, pp. 116620. Date of Electronic Publication: 2024 Jul 31.
DOI: 10.1016/j.bios.2024.116620
Abstrakt: The possibility to print electronics by means of office tools has remarkedly increased the possibility to design affordable and robust point-of-care/need devices. However, conductive inks suffer from low electrochemical and rheological performances limiting their applicability in biosensors. Herein, a fast CO 2 laser approach to activate printed carbon inks towards direct enzymatic bioelectrocatalysis (3 rd generation) is proposed and exploited to build biosensors for D-fructose analysis in biological fluids. The CO 2 laser treatment was compared with two lab-grade printed transducers fabricated with solvent (SB) and water (WB) based carbon inks. The use of the laser revealed significant morpho-chemical variations on the printed inks and was investigated towards enzymatic direct catalysis, using Fructose dehydrogenase (FDH) integrated into entirely lab-produced biosensors. The laser-driven activation of the inks unveils the inks' direct electron transfer (DET) ability between FDH and the electrode surface. Sub-micromolar limits of detection (SB-ink LOD = 0.47 μM; WB-ink LOD = 0.24 μM) and good linear ranges (SB-ink: 5-100 μM; WB-ink: 1-50 μM) were obtained, together with high selectivity due to use of the enzyme and the low applied overpotential (0.15 V vs. pseudo-Ag/AgCl). The laser-activated biosensors were successfully used for D-fructose determination in complex synthetic and real biological fluids (recoveries: 93-112%; RSD ≤8.0%, n = 3); in addition, the biosensor ability for continuous measurement (1.5h) was also demonstrated simulating physiological D-fructose fluctuations in cerebrospinal fluid.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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