TNF reduces osteogenic cell fate in PDL cells at transcriptional and functional levels without alteration of periodontal proliferative capacity.

Autor: Knaup I; Department of Orthodontics, Medical Faculty, RWTH Aachen University, Pauwelsstr. 30, 52074, Aachen, Germany. iknaup@ukaachen.de., Kramann R; Clinic for Renal and Hypertensive Disorders, Rheumatological and Immunological Diseases (Medical Clinic II), Medical Faculty, RWTH Aachen University, Aachen, Germany., Sasula MJ; Department of Gastroenterology, Hepatology and Transplant Medicine, Medical Faculty, University of Duisburg-Essen, Essen, Germany., Mack P; Department of Orthodontics, Medical Faculty, RWTH Aachen University, Pauwelsstr. 30, 52074, Aachen, Germany., Bastos Craveiro R; Department of Orthodontics, Medical Faculty, RWTH Aachen University, Pauwelsstr. 30, 52074, Aachen, Germany., Niederau C; Department of Orthodontics, Medical Faculty, RWTH Aachen University, Pauwelsstr. 30, 52074, Aachen, Germany., Coenen F; Department of Orthodontics, Medical Faculty, RWTH Aachen University, Pauwelsstr. 30, 52074, Aachen, Germany., Neuss S; Helmholtz Institute for Biomedical Engineering, BioInterface Group, RWTH Aachen University, Aachen, Germany.; Institute of Pathology, Medical Faculty, RWTH Aachen University, Aachen, Germany., Jankowski J; Institute for Molecular Cardiovascular Research, Medical Faculty, RWTH Aachen University, Aachen, Germany., Wolf M; Department of Orthodontics, Medical Faculty, RWTH Aachen University, Pauwelsstr. 30, 52074, Aachen, Germany.
Jazyk: angličtina
Zdroj: Journal of orofacial orthopedics = Fortschritte der Kieferorthopadie : Organ/official journal Deutsche Gesellschaft fur Kieferorthopadie [J Orofac Orthop] 2024 Aug 02. Date of Electronic Publication: 2024 Aug 02.
DOI: 10.1007/s00056-024-00541-2
Abstrakt: Aims: To investigate the effect of tumor necrosis factor (TNF) on the growth of human periodontal ligament (PDL) cells, their osteogenic differentiation and modulation of their matrix secretion in vitro.
Methods: The influence of 10 ng/ml TNF on proliferation and metabolic activity of PDL cells was analyzed by cell counting (DAPI [4',6-diamidino-2-phenylindole] staining) and the MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay. In addition, cells were cultured under control conditions and osteogenic conditions (media containing 10 mM β-glycerophosphate). Quantitative expression analysis of genes encoding the osteogenic markers alkaline phosphatase (ALP), collagen type I alpha 1 chain (COL1A1), osteoprotegerin (OPG), and osteopontin (OPN) was performed after 7 and 14 days of cultivation. Calcium deposits were stained with alizarin red.
Results: Our studies showed that 10 ng/ml TNF did not affect the survival and metabolic activity of PDL cells. Quantitative expression analysis revealed that long-term cultures with TNF impaired osteogenic cell fate at early and late developmental stages. Furthermore, TNF significantly reduced matrix secretion in PDL cells.
Conclusion: The present data confirm TNF as a regulatory factor of proinflammatory remodeling that influences the differentiation behavior but not the metabolism and cell proliferation of the periodontium. Therefore, TNF represents an interesting target for the regulation of orthodontic remodeling processes in the periodontium.
(© 2024. The Author(s).)
Databáze: MEDLINE
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