| Autor: |
Wang C; College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China., Cheng Y; College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China., Yin X; College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China., Wu Q; College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China., Ma J; College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China., Zhang Q; College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China., Zhao L; Yantai Key Laboratory of Nanoscience and Technology for Prepared Food, Yantai Engineering Research Center of Green Food Processing and Quality Control, College of Food Engineering, Ludong University, Yantai, Shandong 264025, China.; Bio-Nanotechnology Research Institute, Ludong University, Yantai, Shandong 264025, China., Wang J; College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China., Zhang D; College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China.; Yantai Key Laboratory of Nanoscience and Technology for Prepared Food, Yantai Engineering Research Center of Green Food Processing and Quality Control, College of Food Engineering, Ludong University, Yantai, Shandong 264025, China. |
| Abstrakt: |
Conventional lateral flow immunoassay (LFIA) usually suffers from poor antimatrix interference, unsatisfactory sensitivity, and lack of quantitative ability for target analyte detection in food matrices. In response to these limits, here, multifunctional nanomaterial ZnFe 2 O 4 nanoparticles (ZFOs) were developed and integrated into LFIA for powerful magnetic separation/enrichment and colorimetric/photothermal target sensing. Under optimum conditions, the detection for clenbuterol (CL) with magnetic enrichment achieves 9-fold higher sensitivity compared to that without enrichment and 162-fold higher sensitivity compared to that based on traditional colloidal golds. Attributing the improved performances of ZFOs, CL can be detected at ultralow levels in pork and milk with 10 min of immunoreaction time. The vLODs were 0.01 μg kg -1 for two modes, and the cutoff values of CL were about 5 and 3 μg kg -1 , respectively. More importantly, the enrichment ZFO-mediated LFIA (ZE-LFIA) exhibits a similar limit of detection (LOD) in both buffer solution and food matrix, demonstrating a universal resistance to the food matrix. The multitudinous performance merits of this ZE-LFIA with high sensitivity, matrix tolerance, accuracy, and specificity have ensured a broad application potential for target detection of clenbuterol and can serve as an experience for other veterinary drug residues' detection. |
