Probiotic Limosilactobacillus reuteri KUB-AC5 decreases urothelial cell invasion and enhances macrophage killing of uropathogenic Escherichia coli in vitro study.

Autor:	Tantibhadrasapa A; Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand., Li S; Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.; Key Laboratory of Tumor Immunopathology, Youjiang Medical University for Nationalities, Baise, China., Buddhasiri S; Research Center for Veterinary Biosciences and Veterinary Public Health, Chiang Mai University, Chiang Mai, Thailand.; Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, Thailand., Sukjoi C; Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand., Mongkolkarvin P; Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand., Boonpan P; Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand., Wongpalee SP; Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand., Paenkaew P; Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand., Sutheeworapong S; Pilot Plant Development and Training Institute (PDTI), King Mongkut's University of Technology Thonburi (KMUTT), Bangkok, Thailand., Nakphaichit M; Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok, Thailand.; Specialized Research Unit: Probiotics and Prebiotics for Health, Faculty of Agro-Industry, Kasetsart University, Bangkok, Thailand., Nitisinprasert S; Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok, Thailand.; Specialized Research Unit: Probiotics and Prebiotics for Health, Faculty of Agro-Industry, Kasetsart University, Bangkok, Thailand., Hsieh MH; Department of Urology, School of Medicine and Health Sciences, The George Washington University, Washington, DC, United States.; Department of Pediatrics, School of Medicine and Health Sciences, The George Washington University, Washington, DC, United States.; Department of Microbiology, Immunology, and Tropical Medicine, School of Medicine and Health Sciences, The George Washington University, Washington, DC, United States., Thiennimitr P; Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.; Research Center of Microbial Diversity and Sustainable Utilization, Chiang Mai University, Chiang Mai, Thailand.; Center of Multidisciplinary Technology for Advanced Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
Jazyk:	angličtina
Zdroj:	Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2024 Jul 18; Vol. 14, pp. 1401462. Date of Electronic Publication: 2024 Jul 18 (Print Publication: 2024).
DOI:	10.3389/fcimb.2024.1401462
Abstrakt:	Introduction: Bacterial urinary tract infections (UTI) are among the most common infectious diseases worldwide. The rise of multidrug-resistant (MDR) uropathogenic Escherichia coli (UPEC) UTI cases is a significant threat to healthcare systems. Several probiotic bacteria have been proposed as an alternative to combat MDR UTI. Lactic acid bacteria in the genus Limosilactobacillus are some of the most studied and used probiotics. However, strain-specific effects play a critical role in probiotic properties. L. reuteri KUB-AC5 (AC5), isolated from the chicken gut, confers antimicrobial and immunobiotic effects against some human pathogens. However, the antibacterial and immune modulatory effects of AC5 on UPEC have never been explored. Methods: Here, we investigated both the direct and indirect effects of AC5 against UPEC isolates (UTI89, CFT073, and clinical MDR UPEC AT31) in vitro . Using a spot-on lawn, agar-well diffusion, and competitive growth assays, we found that viable AC5 cells and cell-free components of this probiotic significantly reduced the UPEC growth of all strains tested. The human bladder epithelial cell line UM-UC-3 was used to assess the adhesion and pathogen-attachment inhibition properties of AC5 on UPEC. Results and Discussion: Our data showed that AC5 can attach to UM-UC-3 and decrease UPEC attachment in a dose-dependent manner. Pretreatment of UPEC-infected murine macrophage RAW264.7 cells with viable AC5 (multiplicity of infection, MOI = 1) for 24 hours enhanced macrophage-killing activity and increased proinflammatory ( Nos2 , Il6 , and Tnfa ) and anti-inflammatory ( Il10 ) gene expression. These findings indicate the gut-derived AC5 probiotic could be a potential urogenital probiotic against MDR UTI. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision. (Copyright © 2024 Tantibhadrasapa, Li, Buddhasiri, Sukjoi, Mongkolkarvin, Boonpan, Wongpalee, Paenkaew, Sutheeworapong, Nakphaichit, Nitisinprasert, Hsieh and Thiennimitr.)
Databáze:	MEDLINE
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