Progression from in vivo validation to in vitro screening in hazard assessment for leukoderma-inducible chemicals.

Autor: Tazaki A; Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan; Activities of the Institute of Innovation for Future Society of Nagoya University, Japan; Voluntary Body for International Healthcare in Universities, Nagoya, Aichi, Japan., Nishadhi DASM; Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan; Voluntary Body for International Healthcare in Universities, Nagoya, Aichi, Japan., Li A; Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan; Voluntary Body for International Healthcare in Universities, Nagoya, Aichi, Japan., Zhang L; Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan., Maw TH; Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan; Voluntary Body for International Healthcare in Universities, Nagoya, Aichi, Japan., Kondo-Ida L; Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan; Department of Molecular and Cancer Medicine, Faculty of Pharmacy, Meijo University, Nagoya, Japan., Yanagisawa K; Department of Molecular and Cancer Medicine, Faculty of Pharmacy, Meijo University, Nagoya, Japan., Kato M; Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine, Nagoya, Aichi, Japan; Activities of the Institute of Innovation for Future Society of Nagoya University, Japan; Voluntary Body for International Healthcare in Universities, Nagoya, Aichi, Japan. Electronic address: kato.masashi.r6@f.mail.nagoya-u.ac.jp.
Jazyk: angličtina
Zdroj: Environmental pollution (Barking, Essex : 1987) [Environ Pollut] 2024 Sep 01; Vol. 356, pp. 124508. Date of Electronic Publication: 2024 Aug 01.
DOI: 10.1016/j.envpol.2024.124508
Abstrakt: Chemicals are representative environmental factors that affect human health. Recently, external exposure to a chemical of rhododenol (RD) caused chemical leukoderma, an acquired patchy hypopigmentation, in about 20,000 Asian people. The development of a hazard assessment system for accurate determination of leukoderma-inducible chemicals is required for the prevention of such tragedies. Case studies in humans have shown 6 chemicals, including RD, with a constitutive leukoderma-inducible potency and 3 chemicals with a photosensitive but not a constitutive leukoderma-inducible potency. In this study, the 6 positive and 3 negative control chemicals with or without constitutive leukoderma-inducible potencies were investigated by our previously developed in vivo hazard assessment system using tail skin of mice. Based on the results of validation, this study aimed to develop an in vitro hazard assessment system to correctly determine chemicals with a constitutive leukoderma-inducible potency. As expected, external exposure to the 6 positive control chemicals, but not external exposure to the 3 negative control chemicals, resulted in development of constitutive leukoderma in mouse tail skin with a decreased level of skin melanin and decreased number of melanocytes. Moreover, the 6 positive and 3 negative control chemicals were correctly distinguished by the presence or absence of endoplasmic reticulum (ER) stress induction, but not by tyrosinase-dependent cell death or production of reactive oxygen species (ROS), in immortalized normal melanocytes. The hazard assessment system using tail skin could be a solid in vivo tool to reliably determine the chemical potency of a chemical for constitutive leukoderma induction. The hazard assessment system focusing on ER stress induction in normal melanocytes might be a novel and convenient in vitro tool for accurately evaluating chemicals with leukoderma-inducible potencies. Thus, this study contributed to environmentology through the development of a screening system for preventing an environmental factor-related disease.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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