Elevated temperature impairs gonadal development by suppressing the expression of the genes for kisspeptin, GnRH1 and GTH subunits in Nile tilapia Oreochromis niloticus.

Autor: Naziat A; Department of Fish Biology and Biotechnology, Chattogram Veterinary and Animal Sciences University, Chattogram 4225, Bangladesh., Islam SMM; Laboratory of Fish Ecophysiology, Department of Fisheries Management, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh., Chakrabarty J; Department of Fish Biology and Biotechnology, Chattogram Veterinary and Animal Sciences University, Chattogram 4225, Bangladesh., Paray BA; Department of Zoology, College of Science, King Saud University, PO Box 2455, Riyadh 11451, Saudi Arabia., Zahangir MM; Department of Fish Biology and Biotechnology, Chattogram Veterinary and Animal Sciences University, Chattogram 4225, Bangladesh., Ando H; Marine Biological Station, Sado Island Center for Ecological Sustainability, Niigata University, Sado, Niigata 952-2135, Japan., Shahjahan M; Laboratory of Fish Ecophysiology, Department of Fisheries Management, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh. Electronic address: mdshahjahan@bau.edu.bd.
Jazyk: angličtina
Zdroj: Comparative biochemistry and physiology. Part A, Molecular & integrative physiology [Comp Biochem Physiol A Mol Integr Physiol] 2024 Nov; Vol. 297, pp. 111714. Date of Electronic Publication: 2024 Jul 30.
DOI: 10.1016/j.cbpa.2024.111714
Abstrakt: Temperature is a preeminent factor in the regulation of fish reproduction and hinders gonadal development beyond a specific threshold. To comprehend the molecular mechanism responsible for reproductive suppression at different temperature, expression of the genes encoding kisspeptin (kiss2), gonadotropin-releasing hormone (gnrh1) and their receptors (gpr54, gnrh1r) in the brain, and the gonadotropin (GTH) subunits (fshb and lhb) in the pituitary were studied in juvenile Nile tilapia (Oreochromis niloticus) along with gonadal histology. Fish were acclimatized to three distinct temperatures, including 31 °C, 34 °C and 37 °C for 14 days. The mRNA levels of kiss2, gpr54, gnrh1, and gnrh1r were significantly decreased at 37 °C compared to 31 °C and 34 °C in the both sexes. In parallel, the expression level of fshb in the both sexes and lhb in the female were significantly lower at 37 °C in the pituitary. Histologically, the gonads of both sexes had normal growth of gametes at control temperature (31 °C), whereas the spermatogenesis and oocyte maturation were slowed down and atretic oocytes were found in the ovary at 37 °C acclimation temperature. Taken together, the results imply that elevated temperature beyond the specific threshold may have a negative impact on reproduction by suppressing the gene expressions of kisspeptin/GnRH1/GTH system and eventually restrains normal growth and maturation of gametes in the both sexes of Nile tilapia.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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