Horizontal plasmid transfer promotes antibiotic resistance in selected bacteria in Chinese frog farms.

Autor: Zhuang M; Faculty of Biotechnology and Food Engineering, Technion-Israel Institute of Technology, Haifa, Israel; Department of Biotechnology and Food Engineering Program, Guangdong Technion-Israel Institute of Technology, Shantou 515063, China., Yan W; Department of Microbiology and Molecular Genetics, The Hebrew University of Jerusalem, POB 12272, Jerusalem 91120, Israel., Xiong Y; Department of Microbiology and Molecular Genetics, The Hebrew University of Jerusalem, POB 12272, Jerusalem 91120, Israel., Wu Z; Department of Microbiology and Molecular Genetics, The Hebrew University of Jerusalem, POB 12272, Jerusalem 91120, Israel., Cao Y; Faculty of Biotechnology and Food Engineering, Technion-Israel Institute of Technology, Haifa, Israel; Department of Biotechnology and Food Engineering Program, Guangdong Technion-Israel Institute of Technology, Shantou 515063, China., Sanganyado E; Department of Applied Sciences, Northumbria University, Newcastle Upon Tyne NE1 8ST, UK., Siame BA; Department of Biology, Trinity Western University, Langley, British Columbia V2Y 1Y1, Canada., Chen L; Department of Computer Science, College of Mathematics and Computer, Shantou University, Shantou 515063, China. Electronic address: chenliang@stu.edu.cn., Kashi Y; Faculty of Biotechnology and Food Engineering, Technion-Israel Institute of Technology, Haifa, Israel., Leung KY; Faculty of Biotechnology and Food Engineering, Technion-Israel Institute of Technology, Haifa, Israel; Department of Biotechnology and Food Engineering Program, Guangdong Technion-Israel Institute of Technology, Shantou 515063, China. Electronic address: kayin.leung@gtiit.edu.cn.
Jazyk: angličtina
Zdroj: Environment international [Environ Int] 2024 Aug; Vol. 190, pp. 108905. Date of Electronic Publication: 2024 Jul 23.
DOI: 10.1016/j.envint.2024.108905
Abstrakt: The emergence and dissemination of antibiotic resistance genes (ARGs) in the ecosystem are global public health concerns. One Health emphasizes the interconnectivity between different habitats and seeks to optimize animal, human, and environmental health. However, information on the dissemination of antibiotic resistance genes (ARGs) within complex microbiomes in natural habitats is scarce. We investigated the prevalence of antibiotic resistant bacteria (ARB) and the spread of ARGs in intensive bullfrog (Rana catesbeiana) farms in the Shantou area of China. Antibiotic susceptibilities of 361 strains, combined with microbiome analyses, revealed Escherichia coli, Edwardsiella tarda, Citrobacter and Klebsiella sp. as prevalent multidrug resistant bacteria on these farms. Whole genome sequencing of 95 ARB identified 250 large plasmids that harbored a wide range of ARGs. Plasmid sequences and sediment metagenomes revealed an abundance of tetA, sul1, and aph(3″)-Ib ARGs. Notably, antibiotic resistance (against 15 antibiotics) highly correlated with plasmid-borne rather than chromosome-borne ARGs. Based on sequence similarities, most plasmids (62%) fell into 32 distinct groups, indicating a potential for horizontal plasmid transfer (HPT) within the frog farm microbiome. HPT was confirmed in inter- and intra-species conjugation experiments. Furthermore, identical mobile ARGs, flanked by mobile genetic elements (MGEs), were found in different locations on the same plasmid, or on different plasmids residing in the same or different hosts. Our results suggest a synergy between MGEs and HPT to facilitate ARGs dissemination in frog farms. Mining public databases retrieved similar plasmids from different bacterial species found in other environmental niches globally. Our findings underscore the importance of HPT in mediating the spread of ARGs in frog farms and other microbiomes of the ecosystem.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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