Polyethylene terephthalate nanoparticles induce oxidative damage in Chlorella vulgaris.

Autor: Vijayan S; Department of Botany, Fatima Mata National College, Kollam, Kerala, India, 691001., Liu R; Department of Food Science and Agricultural Chemistry, MacDonald Campus, McGill University, 21111 Lakeshore Ste Anne de Bellevue, Quebec, H9X3V9, Canada., George S; Department of Food Science and Agricultural Chemistry, MacDonald Campus, McGill University, 21111 Lakeshore Ste Anne de Bellevue, Quebec, H9X3V9, Canada., Bhaskaran S; Department of Botany, Fatima Mata National College, Kollam, Kerala, India, 691001. Electronic address: sinilalb@fatimacollege.net.
Jazyk: angličtina
Zdroj: Plant physiology and biochemistry : PPB [Plant Physiol Biochem] 2024 Oct; Vol. 215, pp. 108987. Date of Electronic Publication: 2024 Jul 31.
DOI: 10.1016/j.plaphy.2024.108987
Abstrakt: Polyethylene Terephthalate (PET) is a type of plastic largely used for packing food and beverages. Unfortunately, it includes a major portion of the plastic distributed through aquatic systems wherever systematic collection and recycling are lacking. Although PET is known to be non-toxic, it is not obvious whether the nanoparticles (NPs) formed due to their degradation have any direct/indirect effect on aquatic organisms. In order to study the effects on aquatic environment, fresh water algae Chlorella vulgaris was subjected to incremental concentrations of the NPs. We observed a concentration and duration of exposure dependent decrease in algal growth rate along with reduced total chlorophyll content. Scanning electron microscopy revealed deformities in cell shape and the uptake of Propidium Iodide suggested membrane damage in response to NP exposure. Intracellular Reactive Oxygen Species level was also found significantly higher, evidenced by Dichlorodihydrofluorescein diacetate staining. Activity of antioxidant enzymes Superoxide dismutase (SOD), Peroxidase (POD) and Catalase (CAT) were significantly higher in the NP exposed groups suggesting the cellular response to regain homeostasis. Further, expression levels of the genes psaB, psbC, and rbcL associated with photosynthesis increased above two fold with respect to the control inferring the possibility of damage to photosynthesis and the initial molecular responses to circumvent the situation. In short, our studies provide evidence for oxidative stress mediated cellular damages in Chlorella vulgaris exposed to NPs of PET.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Masson SAS. All rights reserved.)
Databáze: MEDLINE
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