Optimization of the flow cytometry method of detection, quantification and qualification of microorganisms in carrot juice.

Autor: Ratajczak K; Department of Food Technology of Plant Origin, Poznan University of Life Sciences, Wojska Polskiego 31, 60-624 Poznań, Poland. Electronic address: katherine.ratajczak@gmail.com., Juzwa W; Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Wojska Polskiego 48, 60-627 Poznań, Poland. Electronic address: wojciech.juzwa@up.poznan.pl., Piotrowska-Cyplik A; Department of Food Technology of Plant Origin, Poznan University of Life Sciences, Wojska Polskiego 31, 60-624 Poznań, Poland. Electronic address: agnieszka.piotrowska-cyplik@up.poznan.pl.
Jazyk: angličtina
Zdroj: Food chemistry [Food Chem] 2024 Dec 01; Vol. 460 (Pt 2), pp. 140606. Date of Electronic Publication: 2024 Jul 24.
DOI: 10.1016/j.foodchem.2024.140606
Abstrakt: Fresh, unpasteurized carrot juice is a popular element of the everyday diet of many consumers, and as such the matter of the juice's microbial safety remains an important one. Imaging flow cytometry (FCM) allows a fast enumeration and determination of cells, as well as their further differentiation. However, carrot juice is a difficult food product to analyze with the use of FCM due to interference from autofluorescence and the presence of plant debris. In this research, we aimed to obtain an effective and repeatable protocol for the preparation of carrot juice samples for FCM analysis. Through experimental and software-based means we successfully determined a reliable protocol for the preparation of fresh, unpasteurized carrot juice, which consisted of a sequence of filtering, centrifugation, enzyme treatment, and finally the implementation of the Machine Learning protocol for the best result.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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