Setting epidemiological cut-off values relevant to MIC and disc diffusion data for Aeromonas salmonicida generated by a standard method.

Autor:	Smith P; School of Natural Science, University of Galway, Galway H91 TK33, Ireland., Buba E; Institute of Aquaculture, Faculty of Natural Sciences, University of Stirling, Stirling FK9 4LA, UK.; Veterinary Medicines Directorate (VMD), Woodham Lane, New Haw, Surrey KT15 3LS, UK., Desbois AP; Institute of Aquaculture, Faculty of Natural Sciences, University of Stirling, Stirling FK9 4LA, UK., Adams A; Institute of Aquaculture, Faculty of Natural Sciences, University of Stirling, Stirling FK9 4LA, UK., Verner-Jeffreys D; Centre for Environment, Fisheries and Aquaculture Science, Weymouth DT4 8UB, UK.; UK FAO Reference Centre for AMR, Weymouth DT4 8UB, UK., Joseph A; Centre for Environment, Fisheries and Aquaculture Science, Weymouth DT4 8UB, UK.; UK FAO Reference Centre for AMR, Weymouth DT4 8UB, UK., Light E; Veterinary Medicines Directorate (VMD), Woodham Lane, New Haw, Surrey KT15 3LS, UK., Le Devendec L; Anses, Ploufragan-Plouzané-Niort Laboratory, Mycoplasmology-Bacteriology-Antibioresistance Unit, 22440 Ploufragan, France., Jouy E; Anses, Ploufragan-Plouzané-Niort Laboratory, Mycoplasmology-Bacteriology-Antibioresistance Unit, 22440 Ploufragan, France., Larvor E; Anses, Ploufragan-Plouzané-Niort Laboratory, Mycoplasmology-Bacteriology-Antibioresistance Unit, 22440 Ploufragan, France., Boitard PM; FILI@VET, 29600 Saint Martin des Champs, France., Jamin M; FILI@VET, 29600 Saint Martin des Champs, France., Keck N; Laboratoire Départemental Vétérinaire de l'Hérault, 34000 Montpellier, France., Le Breton A; Selarl Vet'Eau, 31330 Grenade sur Garonne, France., Thuillier B; Labocéa, Microbiologie vétérinaire, site de Quimper, 29000 Quimper, France., Ravaille C; SOCSA Elevage, 81300 Graulhet, France., Morin T; Anses, Ploufragan-Plouzané-Niort Laboratory, Fish Virology, Immunology and Ecotoxicology Unit (VIMEP), 29280 Plouzané, France., Baron S; Anses, Ploufragan-Plouzané-Niort Laboratory, Mycoplasmology-Bacteriology-Antibioresistance Unit, 22440 Ploufragan, France.
Jazyk:	angličtina
Zdroj:	Diseases of aquatic organisms [Dis Aquat Organ] 2024 Aug 01; Vol. 159, pp. 29-35. Date of Electronic Publication: 2024 Aug 01.
DOI:	10.3354/dao03798
Abstrakt:	The Clinical and Laboratory Standards Institute has published epidemiological cut-off values for susceptibility data generated at 22°°C and read after 44-48 h for florfenicol, oxolinic acid and oxytetracycline against Aeromonas salmonicida. The cut-off values for the minimum inhibitory concentration (MIC) and disc diffusion were derived from data obtained by 1 laboratory and 2 laboratories respectively. The present work reports the generation of susceptibility data from additional laboratories and the calculation of provisional cut-off values from aggregations of these data with previously published data. With respect to MIC data, the provisional cut-off values, derived from aggregations of the data from 4 laboratories, were ≤4 µg ml-1 for florfenicol, ≤0.0625 µg ml-1 for oxolinic acid and ≤1 µg ml-1 for oxytetracycline. For disc diffusion data, the provisional cut-off values derived from aggregations of the data from 5 laboratories were ≥30 mm for florfenicol, ≥32 mm for oxolinic acid and ≥25 mm for oxytetracycline. In addition, a cut-off value of ≥29 mm for ampicillin was derived from the aggregation of data from 4 laboratories.
Databáze:	MEDLINE
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