Expression patterns of immune checkpoint proteins and Plasmodium falciparum -induced cytokines in chronic hepatitis B virus-infected and uninfected individuals: A cross-sectional study.

Autor:	Segbefia SP; Department of Immunology, NMIMR, College of Health Sciences University of Ghana Accra Ghana.; Department of Molecular Medicine, School of Medicine and Dentistry College of Health Sciences, KNUST Kumasi Ghana., Asandem DA; Department of Biochemistry, Cell and Molecular Biology, West African Centre for Cell Biology of Infectious Pathogens, College of Basic and Applied Sciences University of Ghana Accra Ghana.; Department of Virology, NMIMR, College of Health Sciences University of Ghana Accra Ghana., Pobee A; Department of Immunology, NMIMR, College of Health Sciences University of Ghana Accra Ghana., Asare B; Department of Immunology, NMIMR, College of Health Sciences University of Ghana Accra Ghana.; Department of Animal Biology and Conservation Science, College of Basic and Applied Sciences University of Ghana Accra Ghana., Prah AD; Department of Biochemistry, Cell and Molecular Biology, West African Centre for Cell Biology of Infectious Pathogens, College of Basic and Applied Sciences University of Ghana Accra Ghana., Baba-Adam R; Department of Immunology, NMIMR, College of Health Sciences University of Ghana Accra Ghana.; Department of Biochemistry, Cell and Molecular Biology, West African Centre for Cell Biology of Infectious Pathogens, College of Basic and Applied Sciences University of Ghana Accra Ghana., Amponsah JA; Department of Immunology, NMIMR, College of Health Sciences University of Ghana Accra Ghana., Kyei-Baafour E; Department of Immunology, NMIMR, College of Health Sciences University of Ghana Accra Ghana., van der Puije W; Department of Immunology, NMIMR, College of Health Sciences University of Ghana Accra Ghana., Osei F; Department of Immunology, NMIMR, College of Health Sciences University of Ghana Accra Ghana., Teye-Adjei D; Department of Immunology, NMIMR, College of Health Sciences University of Ghana Accra Ghana., Agyemang S; Department of Biochemistry, Cell and Molecular Biology, West African Centre for Cell Biology of Infectious Pathogens, College of Basic and Applied Sciences University of Ghana Accra Ghana., Brenko T; Department of Immunology, NMIMR, College of Health Sciences University of Ghana Accra Ghana., Bentum-Ennin L; Department of Immunology, NMIMR, College of Health Sciences University of Ghana Accra Ghana., Tetteh JKA; Department of Immunology, NMIMR, College of Health Sciences University of Ghana Accra Ghana., Bonney KJH; Department of Virology, NMIMR, College of Health Sciences University of Ghana Accra Ghana., Sakyi SA; Department of Molecular Medicine, School of Medicine and Dentistry College of Health Sciences, KNUST Kumasi Ghana., Amoah LE; Department of Immunology, NMIMR, College of Health Sciences University of Ghana Accra Ghana.; Department of Biochemistry, Cell and Molecular Biology, West African Centre for Cell Biology of Infectious Pathogens, College of Basic and Applied Sciences University of Ghana Accra Ghana., Kusi KA; Department of Immunology, NMIMR, College of Health Sciences University of Ghana Accra Ghana.; Department of Biochemistry, Cell and Molecular Biology, West African Centre for Cell Biology of Infectious Pathogens, College of Basic and Applied Sciences University of Ghana Accra Ghana.
Jazyk:	angličtina
Zdroj:	Health science reports [Health Sci Rep] 2024 Jul 29; Vol. 7 (8), pp. e2280. Date of Electronic Publication: 2024 Jul 29 (Print Publication: 2024).
DOI:	10.1002/hsr2.2280
Abstrakt:	Background and Aim: Chronic hepatitis B virus (CHB) infection remains a major public health problem. The American Association for the Study of Liver Diseases (AASLD) 2018 Hepatitis B Guidelines provide that CHB individuals not requiring antiviral therapy yet are monitored to determine the need for antiviral therapy in the future; however, these tests do not include measurement of cytokines and immune cell characterization. This case-control study compared the cytokine and immune checkpoint protein expression profiles between CHB individuals not yet on antiviral treatment and hepatitis B virus (HBV)-negative individuals. Methods: CD4 and CD8 T cells from CHB and HBV-negative individuals were characterized for immune checkpoint proteins programmed cell death-1 (PD1), T cell Immunoglobulin domain and mucin domain-containing protein 3 (TIM-3), and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) (CD152), and a memory marker CXCR3 (CD183) using flow cytometry. Malaria-induced cytokine expression levels were determined by stimulating their blood cells with Plasmodium falciparum 3D7 strain antigens (CSP, AMA-1, and TRAP) in whole blood assays, and cytokine levels were measured using a 13-plex Luminex kit. Results: HBV-negative and CHB individuals had comparable levels of CD4+ and CD8+ T cells. However, a proportion of the CD4+ and CD8+ populations from both groups, which were CXCR3+, expressed PD-1 and CD152. The ability to produce cytokines in response to malaria antigen stimulation was not significantly different between the groups. Conclusion: These findings support excluding CHB individuals from antiviral therapy at this stage of infection. However, CHB individuals require regular monitoring to determine the need for later antiviral treatment. Competing Interests: The authors declare no conflict of interest. (© 2024 The Author(s). Health Science Reports published by Wiley Periodicals LLC.)
Databáze:	MEDLINE
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