Quantitative proteomics reveals significant variation in host responses of cattle with differing buffalo fly susceptibility.

Autor:	Kamran M; The University of Queensland, Queensland Alliance for Agriculture & Food Innovation, Centre for Animal Science, St Lucia, QLD, Australia., Raza A; The University of Queensland, Queensland Alliance for Agriculture & Food Innovation, Centre for Animal Science, St Lucia, QLD, Australia.; University of Copenhagen, Faculty of Health and Medical Sciences, Department of Veterinary and Animal Sciences, Frederiksberg, Denmark., Naseem MN; The University of Queensland, Queensland Alliance for Agriculture & Food Innovation, Centre for Animal Science, St Lucia, QLD, Australia.; The University of Queensland, School of Veterinary Science, Gatton, QLD, Australia., Turni C; The University of Queensland, Queensland Alliance for Agriculture & Food Innovation, Centre for Animal Science, St Lucia, QLD, Australia., Tabor AE; The University of Queensland, Queensland Alliance for Agriculture & Food Innovation, Centre for Animal Science, St Lucia, QLD, Australia.; The University of Queensland, School of Chemistry and Molecular Bioscience, Brisbane, QLD, Australia., James P; The University of Queensland, Queensland Alliance for Agriculture & Food Innovation, Centre for Animal Science, St Lucia, QLD, Australia.
Jazyk:	angličtina
Zdroj:	Frontiers in immunology [Front Immunol] 2024 Jul 16; Vol. 15, pp. 1402123. Date of Electronic Publication: 2024 Jul 16 (Print Publication: 2024).
DOI:	10.3389/fimmu.2024.1402123
Abstrakt:	Background: Control of buffalo flies ( Haematobia irritans exigua , BFs) relies mainly on chemical methods; however, resistance to insecticides is widespread in BF populations. Breeding for resistance to BFs represents a possible alternative, but direct phenotyping of animals is laborious and often inaccurate. The availability of reliable diagnostic biomarker(s) to identify low BF carrier cattle would facilitate rapid and accurate selection for genetic improvement. However, limited information is available regarding differences amongst cattle in host responses to BF infestation. Methods: This study investigated the variation in Brangus cattle serum proteomic profiles before (naïve) and after peak BF exposure, in low (LF) and high BF burden (HF) cattle. Cattle were phenotyped for susceptibility based on BF counts on multiple dates using visual and photographic techniques. The relative abundance of serum proteins in cattle before and after exposure to BFs was analysed using sequential window acquisition of all theoretical fragment ion mass spectrometry (SWATH-MS). Results: Exposure to BFs elicited similar responses in HF and LF cattle, with 79 and 70 proteins, respectively, showing significantly different abundances post exposure as compared to their relevant naïve groups. The comparison of serum samples from naïve HF and LF cattle identified 44 significantly differentially abundant (DA) proteins, while 37 significantly DA proteins were identified from the comparison between HF and LF cattle post-exposure to BFs. Proteins with higher abundance in naïve LF cattle were enriched in blood coagulation mechanisms that were sustained after exposure to BFs. Strong immune response mechanisms were also identified in naïve LF cattle, whereas these responses developed in HF cattle only after exposure to BF. High BF cattle also showed active anticoagulation mechanisms in response to BF exposure, including downregulation of coagulation factor IX and upregulation of antithrombin-III, which might facilitate BF feeding. Conclusion: Underlying differences in the abundance of proteins related to blood coagulation and immune response pathways could potentially provide indirect indicators of susceptibility to BF infestation and biomarkers for selecting more BF-resistant cattle. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision. (Copyright © 2024 Kamran, Raza, Naseem, Turni, Tabor and James.)
Databáze:	MEDLINE
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