Non-thermal obliteration of critically ranked carbapenem-resistant Acinetobacter baumannii and its resistance gene in a batch atmospheric plasma reactor.

Autor: Mosaka TBM; Sustainable Energy and Environment Research Group, Department of Chemical Engineering, Faculty of Engineering, Built Environment and Information Technology, University of Pretoria, Hatfield, 0002, Pretoria, South Africa., Unuofin JO; Sustainable Energy and Environment Research Group, Department of Chemical Engineering, Faculty of Engineering, Built Environment and Information Technology, University of Pretoria, Hatfield, 0002, Pretoria, South Africa. junuofin@gmail.com., Daramola MO; Sustainable Energy and Environment Research Group, Department of Chemical Engineering, Faculty of Engineering, Built Environment and Information Technology, University of Pretoria, Hatfield, 0002, Pretoria, South Africa., Tizaoui C; Water and Resources Recovery Research Lab, Department of Chemical Engineering, Faculty of Science and Engineering, Swansea University, Swansea, SA1 8EN, UK., Iwarere SA; Sustainable Energy and Environment Research Group, Department of Chemical Engineering, Faculty of Engineering, Built Environment and Information Technology, University of Pretoria, Hatfield, 0002, Pretoria, South Africa.
Jazyk: angličtina
Zdroj: Environmental science and pollution research international [Environ Sci Pollut Res Int] 2024 Aug; Vol. 31 (37), pp. 49811-49822. Date of Electronic Publication: 2024 Jul 31.
DOI: 10.1007/s11356-024-34475-4
Abstrakt: Wastewater treatment plants (WWTPs) have been implicated as direct key reservoir of both antibiotic-resistant bacteria (ARB) and antibiotic-resistant genes (ARGs) associated with human infection, as high concentrations of ARBs and ARGs have been detected in recycled hospital wastewater. Among the ARBs, the carbapenem-resistant Acinetobacter baumannii has been ranked as priority 1 (critical) pathogen by the World Health Organization (WHO), due to its overwhelming burden on public health. Therefore, this study is aimed at investigating non-thermal plasma (NTP) technology as an alternative disinfection step to inactivate this bacterium and its ARGs. Culture-based method and PCR were employed in confirming the carbapenem resistance gene bla NDM-1 in A. baumannii (BAA 1605). Suspension of carbapenem-resistant A. baumannii (24 h culture) was prepared from the confirmed isolate and subjected to plasma treatment at varying time intervals (3 min, 6 min, 9 min, 12 min, and 15 min) in triplicates. The plasma-treated samples were evaluated for re-growth and the presence of the resistance gene. The treatment resulted in a 1.13 log reduction after 3 min and the highest log reduction of ≥ 8 after 15 min, and the results also showed that NTP was able to inactivate the bla NDM-1 gene. The log reduction and gel image results suggest that plasma disinfection has a great potential to be an efficient tertiary treatment step for WWTPs.
(© 2024. The Author(s).)
Databáze: MEDLINE
Externí odkaz: