Global lysine succinylation analysis unveils post-translational regulation effect on phenylpropanoid metabolism remodeling during Lonicera japonica flower development.

Autor: Chen Y; College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310018, China., Shan L; College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310018, China., Zheng W; College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310018, China., Chen J; College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310018, China., Deng L; The Third Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, 310005, China., Tian X; College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310018, China., Xie R; College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310018, China., Yang Y; College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310018, China., Zhang L; College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310018, China. Electronic address: zhanglin@zju.edu.cn., Yang B; College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310018, China. Electronic address: xianyb@zju.edu.cn.
Jazyk: angličtina
Zdroj: Plant physiology and biochemistry : PPB [Plant Physiol Biochem] 2024 Oct; Vol. 215, pp. 108978. Date of Electronic Publication: 2024 Jul 27.
DOI: 10.1016/j.plaphy.2024.108978
Abstrakt: Lonicera japonica plays a significant role in traditional Chinese medicine and as a food source, making it a focus of studies on protein succinylation and its potential role in regulating secondary metabolism during flower development. This study aimed to clarify the regulatory mechanism of protein succinylation on phenylpropanoid-related phenotypic changes by conducting a global lysine succinylation proteomic analysis across different flowering stages. A total of 586 lysine succinylated peptides in 303 proteins were identified during early and late floral stages. Functional enrichment analysis revealed that succinylated proteins primarily participated in the tricarboxylic acid (TCA) cycle, amino acid metabolism, and secondary metabolism. The abundance of succinylated aspartate transaminase (AT), 4-coumarate-CoA ligase (4CL), and phenylalanine N-hydroxylase (CYP79A2) in phenylpropanoid metabolism varied during flower development. In vitro experiments demonstrated that succinylation increased AT activity while inhibited 4CL activity. Decreased levels of total flavonoids and phenolic acids indicated significant alterations in phenylpropanoid metabolism during later floral stages. These results suggest that succinylation of TCA cycle proteins not only influences flower development but also, together with AT-4CL-CYP79A2 co-succinylation, redirects phenylpropanoid metabolism during flower development in L. japonica.
Competing Interests: Declaration of competing interest The authors state that there were no known competing financial interests or personal relationships that could have appeared to affect the work reported in this paper.
(Copyright © 2024. Published by Elsevier Masson SAS.)
Databáze: MEDLINE
Externí odkaz: