Molecular study of the transcription factor SKN-1 and its putative relationship with genes that encode GST and antioxidant enzymes in Haemonchus contortus.

Autor: Maza-Lopez J; Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad Universitaria, Ciudad de México 04510, Mexico., Jiménez-Jacinto V; Unidad Universitaria de Secuenciación Masiva y Bioinformática, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Av. Universidad 2001, Chamilpa, Cuernavaca, Morelos 62210, Mexico., Bermúdez-Morales VH; Centro de Investigación sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Av. Universidad ♯655, Col. Santa María Ahuacatitlán, Cuernavaca, Morelos 62100, Mexico., Alonso-Morales RA; Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad Universitaria, Ciudad de México 04510, Mexico., Reyes-Guerrero DE; Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Carr. Fed. Cuernavaca-Cuautla 8534, Jiutepec, Morelos 62574, Mexico., Higuera-Piedrahita RI; Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad Universitaria, Ciudad de México 04510, Mexico., Camas-Pereyra R; Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Carr. Fed. Cuernavaca-Cuautla 8534, Jiutepec, Morelos 62574, Mexico., López-Arellano ME; Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Carr. Fed. Cuernavaca-Cuautla 8534, Jiutepec, Morelos 62574, Mexico. Electronic address: lopez.mariaeugenia@inifap.gob.mx.
Jazyk: angličtina
Zdroj: Veterinary parasitology [Vet Parasitol] 2024 Oct; Vol. 331, pp. 110255. Date of Electronic Publication: 2024 Jul 14.
DOI: 10.1016/j.vetpar.2024.110255
Abstrakt: Haemonchus contortus is a parasitic nematode of ruminants. Once inside its host, it is exposed to reactive oxidative species and responds by synthesising antioxidant enzymes as a defence. In Caenorhabditis elegans, antioxidant genes are regulated by the transcription factor skinhead-1 (Cel-SKN-1). However, there is little information about the function of SKN-1 in H. contortus (Hco-SKN-1). Therefore, we performed a molecular investigation to characterise Hco-SKN-1 and its putative relationship with genes encode antioxidant enzymes, namely glutathione S-transferases (Hco-GSTs, n = 3), superoxide dismutase (Hco-SOD) and catalase (Hco-CAT), which are involved in haematophagy and defence against the host. We used in silico sequence analysis of Hco-SKN-1 and Hco-GSTs to design and perform relative expression assays involving H. contortus eggs, infective larvae (L 3 ) and adults. Furthermore, we exposed H. contortus transitional infective larvae (xL 3 ) to erythrocytes or hydrogen peroxide (H 2 O 2 ) and evaluated the relative expression of antioxidant genes at 24 or 48 h. Gene Ontology (GO) analysis revealed 31 functions associated with Hco-SKN-1 and Hco-GSTs, including stress resistance, larval development and the active immune response. Hco-GST-5957 and Hco-SOD showed the highest expression in adults, indicating a relationship with specific functions at this mature stage. xL 3 exposed to erythrocytes or H 2 O 2 showed significant upregulation of Hco-SKN-1, but it occurred after upregulation of the antioxidant genes, indicating that these genes are not regulated by Hco-SKN-1 during the blood-feeding stage. Additional investigation is necessary to understand the putative regulation of antioxidant genes by Hco-SKN-1 during the blood-feeding stage.
Competing Interests: Declaration of Competing Interest We also state that there is no conflict of interest associated with this second manuscript version and there is not financial or personal relationship with other people or organization that could inappropriately influence our work.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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