Zbtb11 interacts with Otx2 and patterns the anterior neuroectoderm in Xenopus.

Autor: Satou-Kobayashi Y; Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo, Japan.; Advanced Comprehensive Research Organization, Teikyo University, Tokyo, Japan., Takahashi S; Amphibian Research Center, Graduate School of Science, Hiroshima University, Higashi-Hiroshima, Hiroshima, Japan., Haramoto Y; Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan.; Department of Agri-Production Sciences, College of Agriculture, Tamagawa University, Tokyo, Japan., Asashima M; Advanced Comprehensive Research Organization, Teikyo University, Tokyo, Japan., Taira M; Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo, Japan.; Faculty of Science and Engineering, Chuo University, Tokyo, Japan.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2024 Jul 31; Vol. 19 (7), pp. e0293852. Date of Electronic Publication: 2024 Jul 31 (Print Publication: 2024).
DOI: 10.1371/journal.pone.0293852
Abstrakt: The zinc finger and BTB domain-containing 11 gene (zbtb11) is expressed in the Xenopus anterior neuroectoderm, but the molecular nature of the Zbtb11 protein during embryonic development remains to be elucidated. Here, we show the role of Zbtb11 in anterior patterning of the neuroectoderm and the cooperative action with the transcription factor Otx2. Both overexpression and knockdown of zbtb11 caused similar phenotypes: expanded expression of the posterior gene gbx2 in the neural plate, and later microcephaly with reduced eyes, suggesting that a proper level of zbtb11 expression is necessary for normal patterning of the neuroectoderm, including eye formation. Co-immunoprecipitation assays showed that Zbtb11 formed a complex with itself and with a phosphomimetic and repressive form of Otx2, suggesting that Zbtb11 forms a dimer or oligomer and interacts with Otx2 in a phosphorylation-dependent manner. Reporter analysis further showed that Zbtb11 enhanced the activity of the phosphomimetic Otx2 to repress a silencer element of the posterior gene meis3. These data suggest that Zbtb11 coordinates with phosphorylated Otx2 to specify the anterior neuroectoderm by repressing posterior genes.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Satou-Kobayashi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje