CRISPR screens identify genes essential for in vivo virulence among proteins of hyperLOPIT-unassigned subcellular localization in Toxoplasma .

Autor:	Tachibana Y; Department of Immunoparasitology, Research Institute for Microbial Diseases, Osaka, Japan.; Laboratory of Immunoparasitology, WPI Immunology Frontier Research Center, Osaka, Japan., Sasai M; Department of Immunoparasitology, Research Institute for Microbial Diseases, Osaka, Japan.; Laboratory of Immunoparasitology, WPI Immunology Frontier Research Center, Osaka, Japan.; Department of Immunoparasitology, Center for Infectious Disease Education and Research, Osaka University, Osaka, Japan., Yamamoto M; Department of Immunoparasitology, Research Institute for Microbial Diseases, Osaka, Japan.; Laboratory of Immunoparasitology, WPI Immunology Frontier Research Center, Osaka, Japan.; Department of Immunoparasitology, Center for Infectious Disease Education and Research, Osaka University, Osaka, Japan.
Jazyk:	angličtina
Zdroj:	MBio [mBio] 2024 Sep 11; Vol. 15 (9), pp. e0172824. Date of Electronic Publication: 2024 Jul 31.
DOI:	10.1128/mbio.01728-24
Abstrakt:	The research field to identify and characterize genes essential for in vivo virulence in Toxoplasma gondii has been dramatically advanced by a series of in vivo clustered regularly interspaced short palindromic repeats (CRISPR) screens. Although subcellular localizations of thousands of proteins were predicted by the spatial proteomic method called hyperLOPIT, those of more than 1,000 proteins remained unassigned, and their essentiality in virulence was also unknown. In this study, we generated two small-scale gRNA libraries targeting approximately 600 hyperLOPIT-unassigned proteins and performed in vivo CRISPR screens. As a result, we identified several genes essential for in vivo virulence that were previously unreported. We further characterized two candidates, TgGTPase and TgRimM, which are localized in the cytoplasm and the apicoplast, respectively. Both genes are essential for parasite virulence and widely conserved in the phylum Apicomplexa. Collectively, our current study provides a resource for estimating the in vivo essentiality of Toxoplasma proteins with previously unknown localizations.IMPORTANCE Toxoplasma gondii is a protozoan parasite that causes severe infection in immunocompromised patients or newborns. Toxoplasma possesses more than 8,000 genes; however, the genes essential for in vivo virulence were not fully identified. The apicomplexan parasites, including Toxoplasma , developed unique organelles that do not exist in other model organisms; thus, determining the subcellular location of parasite proteins is important for understanding their functions. Here, we used in vivo genetic screens that enabled us to investigate hundreds of genes in Toxoplasma during mouse infection. We screened approximately 600 parasite proteins with previously unknown subcellular localizations. We identified many novel genes that confer parasite virulence in mice. Among the top hits, we characterized two genes essential for in vivo virulence, TgGTPase and TgRimM, which are widely conserved in the phylum Apicomplexa. Our findings will contribute to understanding how apicomplexans adapt to the host environment and cause disease. Competing Interests: The authors declare no conflict of interest.
Databáze:	MEDLINE
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