Ocimum basilicum L. (basil) presents pro-apoptotic activity in an Ehrlich's experimental tumor murine model.

Autor:	Sant'Ana PGDS; Universidade Federal de São João del-Rei - Departamento de Medicina - Laboratório de Patologia Experimental - Divinópolis (MG) - Brazil., Lima WG; Universidade Federal de Minas Gerais - Faculdade de Farmácia - Belo Horizonte (MG) - Brazil., Lopes GFM; Universidade Federal de São João del-Rei - Departamento de Medicina - Laboratório de Patologia Experimental - Divinópolis (MG) - Brazil., Oliveira SE; Universidade Federal de São João del-Rei - Departamento de Ciências Naturais - Laboratório de Patologia Experimental - São João del-Rei (MG) - Brazil., Costa GAFD; Universidade Federal de São João del-Rei - Departamento de Farmácia - Laboratório de Fitoquímica - Divinópolis (MG) - Brazil., Lima LARDS; Universidade Federal de São João del-Rei - Departamento de Farmácia - Laboratório de Fitoquímica - Divinópolis (MG) - Brazil., Ferreira EE; Universidade Federal de São João del-Rei - Departamento de Ciências Naturais - Laboratório de Patologia Experimental - São João del-Rei (MG) - Brazil., Santos ICD; Universidade Federal de São João del-Rei - Departamento de Engenharia de Biossistemas - Laboratório de Biomoléculas e Biofármacos - São João del-Rei (MG) - Brazil., Damázio LCM; Universidade Federal de São João del-Rei - Departamento de Medicina - Laboratório de Reabilitação - São João del-Rei (MG) - Brazil., Ribeiro RIMA; Universidade Federal de São João del-Rei - Departamento de Medicina - Laboratório de Patologia Experimental - Divinópolis (MG) - Brazil., Pinto FCH; Universidade Federal de São João del-Rei - Departamento de Ciências Naturais - Laboratório de Patologia Experimental - São João del-Rei (MG) - Brazil.
Jazyk:	angličtina
Zdroj:	Acta cirurgica brasileira [Acta Cir Bras] 2024 Jul 29; Vol. 39, pp. e393924. Date of Electronic Publication: 2024 Jul 29 (Print Publication: 2024).
DOI:	10.1590/acb393924
Abstrakt:	Purpose: This study aimed to evaluate the therapeutic effect of an ethanol extract of Ocimum basilicum L. (EEOb) aerial parts against Ehrlich's experimental tumor (EET) in mice. Methods: Swiss mice were divided into two groups (control and treated; n = 6). On day 21, all mice were inoculated subcutaneously with 2 × 106 (0.05 mL) EET cells in the left paw for solid tumor development. This study lasted 28 days. Treatment began 24 hours after inoculation with EET. Measurements of dorsoplantar thickness were used to assess tumor growth. The paw pad was collected for histopathological analysis and stained using the argyrophilic nucleolar organizing regions (AgNOR) technique and immunohistochemistry for proliferating cell nuclear antigen, Bcl-2 and Bax. Results: The treatment of animals with EEOb at 100 mg/kg intraperitoneally was able to reduce the growth (Control = 3.7 ± 0.1 mm vs. EEOb = 5.7 ± 0.2 mm) and the number of AgNORs of solid Ehrlich tumor. The antitumor effect of EEOb was associated with the induction of apoptosis of tumoral cell, as suggested by the reduction of the content of Bcl-2 induced by extract. Conclusions: The study demonstrated that daily administration of EEOb is able to reduce the growth of EET by induce apoptosis of tumoral cells.
Databáze:	MEDLINE
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