Using Quality by Design Tools to Study Gel Formulation from Brassica juncea Leaves and Conducting its In vitro, In vivo, Molecular Docking, and ADMET Analyses.

Autor:	Kanakal MM; Faculty of Pharmacy, Quest International University, Ipoh, Perak, Malaysia., Abbas SA; Faculty of Pharmacy, Quest International University, Ipoh, Perak, Malaysia., Khan A; Faculty of Pharmacy, Quest International University, Ipoh, Perak, Malaysia., Sultana S; Shadan Womens' College of Pharmacy, Hyderabad, 500004, Telangana State, India.; Yenepoya Pharmacy College and Research Centre, Yenepoya (Deemed to be University), Mangalore, 575018, Karnataka, India., Fatima H; Yenepoya Pharmacy College and Research Centre, Yenepoya (Deemed to be University), Mangalore, 575018, Karnataka, India.; Deccan School of Pharmacy, Osmania University, Hyderabad, 500001, Telangana, India., Tabasssum R; Shadan Womens' College of Pharmacy, Hyderabad, 500004, Telangana State, India.; Yenepoya Pharmacy College and Research Centre, Yenepoya (Deemed to be University), Mangalore, 575018, Karnataka, India., Ahmed MG; Yenepoya Pharmacy College and Research Centre, Yenepoya (Deemed to be University), Mangalore, 575018, Karnataka, India., Sultana R; Yenepoya Pharmacy College and Research Centre, Yenepoya (Deemed to be University), Mangalore, 575018, Karnataka, India., Mohammed JS; Sri Indu Institute of Pharmacy, Sheriguda(V), Ibrahimpatnam (M), R.R District, Hyderabad, 501510, Telangana State, India., Salfi R; Yenepoya Pharmacy College and Research Centre, Yenepoya (Deemed to be University), Mangalore, 575018, Karnataka, India.; Deccan School of Pharmacy, Osmania University, Hyderabad, 500001, Telangana, India.
Jazyk:	angličtina
Zdroj:	Anti-inflammatory & anti-allergy agents in medicinal chemistry [Antiinflamm Antiallergy Agents Med Chem] 2024; Vol. 23 (3), pp. 187-204.
DOI:	10.2174/0118715230309053240718122527
Abstrakt:	Introduction: This research aims to create a gel formulation of Brassica juncea leaf extract and assess its anti-inflammatory properties using an in silico study. The anti-inflammatory activity has been compared with Diclofenac molecules in PDB id: 4Z69. Further, the Absorption, Distribution, Metabolism, Excretion, and Toxicity analysis has been performed to ensure the therapeutic potential and safety of the drug development process. The Quality by Design tool has been applied to optimize formulation development. Methods: The extracted gel is characterized by performing Fourier transformer infrared, zeta potential, particle size, Scanning Electron Microscope, and entrapment efficiency. Further, the formulation is evaluated by examining its viscosity, spreadability, and pH measurement. An In vitro study of all nine extract suspensions was conducted to determine the drug contents at 276 nm. Results: The optimized suspension has shown the maximum percentage of drug release (82%) in 10 hours of study. Animal study for anti-inflammatory activity was performed, and results of all five groups of animals compared the % inhibition of paw edema at three hours; gel (56.70%), standard (47.86%), and (39.72%) were found. Conclusion: The research could conclude that the anti-inflammatory activity of gel formulation is high compared to extract, and a molecular docking study validates the anti-inflammatory therapeutic effects. ADMET analysis ensures the therapeutic effects and their safety. (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)
Databáze:	MEDLINE
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