| Autor: |
Bollu T; Department of Neurobiology and Behavior, Cornell University, Ithaca, New York, United States., Whitehead SC; Department of Physics, Cornell University, Ithaca, New York, United States., Prasad N; Department of Neurobiology and Behavior, Cornell University, Ithaca, New York, United States., Walker J; Department of Neurobiology and Behavior, Cornell University, Ithaca, New York, United States., Shyamkumar N; Department of Neurobiology and Behavior, Cornell University, Ithaca, New York, United States., Subramaniam R; Department of Neurobiology and Behavior, Cornell University, Ithaca, New York, United States., Kardon B; Department of Neurobiology and Behavior, Cornell University, Ithaca, New York, United States., Cohen I; Department of Physics, Cornell University, Ithaca, New York, United States., Goldberg JH; Department of Neurobiology and Behavior, Cornell University, Ithaca, New York, United States. |
| Abstrakt: |
Holding still and aiming reaches to spatial targets may depend on distinct neural circuits. Using automated homecage training and a sensitive joystick, we trained freely moving mice to contact a joystick, hold their forelimb still, and then reach to rewarded target locations. Mice learned the task by initiating forelimb sequences with clearly resolved submillimeter-scale micromovements followed by millimeter-scale reaches to learned spatial targets. Hundreds of thousands of trajectories were decomposed into millions of kinematic submovements, while photoinhibition was used to test roles of motor cortical areas. Inactivation of both caudal and rostral forelimb areas preserved the ability to produce aimed reaches, but reduced reach speed. Inactivation specifically of contralateral caudal forelimb area (CFA) additionally impaired the ability to aim corrective submovements to remembered locations following target undershoots. Our findings show that motor cortical inactivations reduce the gain of forelimb movements but that inactivation specifically of contralateral CFA impairs corrective movements important for reaching a target location. NEW & NOTEWORTHY To test the role of different cortical areas in holding still and reaching to targets, this study combined home-cage training with optogenetic silencing as mice engaged in a learned center-out-reach task. Inactivation specifically of contralateral caudal forelimb area (CFA) impaired corrective movements necessary to reach spatial targets to earn reward. |
