Autor: |
Das S; Department of Biological Sciences, Indian Institute of Science Education and Research Kolkata, Nadia, Mohanpur, West Bengal 741246, India., Das S; Department of Biological Sciences, Indian Institute of Science Education and Research Kolkata, Nadia, Mohanpur, West Bengal 741246, India., Rath PP; School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India., Banerjee A; Department of Biological Sciences, Indian Institute of Science Education and Research Kolkata, Nadia, Mohanpur, West Bengal 741246, India., Gourinath S; School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India., Mukhopadhyay AK; National Institute of Cholera and Enteric Disease, Kolkata 700010, India., Maiti S; Department of Biological Sciences, Indian Institute of Science Education and Research Kolkata, Nadia, Mohanpur, West Bengal 741246, India. |
Abstrakt: |
Vibrio cholerae ( V. cholerae ), the etiological agent of cholera, employs various virulence factors to adapt and thrive within both aquatic and human host environments. Among these factors, the type VI secretion system (T6SS) stands out as one of the crucial determinants of its pathogenicity. Valine glycine repeat protein G1 (VgrG1) and hemolysin coregulated protein (HCP) are considered major effector molecules of T6SS. Previous studies have highlighted that VgrG1 interacts with HCP proteins. Additionally, it has been shown that VgrG1 possesses an actin cross-linking domain (ACD) with actin-binding activity. Interestingly, it was reported that purified HCP protein treatment increased the stress fibers within cells. Therefore, we hypothesize that HCP may interact with host cell actin, potentially playing a role in the cytoskeletal rearrangement during V. cholerae infection. To test this hypothesis, we characterized HCP from the V. cholerae O139 serotype and demonstrated its interaction with actin monomers. In silico analysis and experimental validation revealed the presence of an actin-binding site within HCP. Furthermore, overexpression of HCP resulted in its colocalization with actin stress fibers in host cells. Our findings establish HCP as an effector molecule for potent host cell actin cytoskeleton remodeling during V. cholerae infection, providing new insights into bacterial pathogenicity mechanisms. Understanding the interplay between bacterial effectors and host cell components is crucial for developing targeted therapeutic interventions against cholera and related infectious diseases. |