| Autor: |
Zhang Y; College of Plant Sciences, State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases/Key Laboratory of Zoonosis Research, Ministry of Education, Jilin University, Changchun 130062, China., Zhang K; College of Plant Sciences, State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases/Key Laboratory of Zoonosis Research, Ministry of Education, Jilin University, Changchun 130062, China., Bao Z; College of Plant Sciences, State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases/Key Laboratory of Zoonosis Research, Ministry of Education, Jilin University, Changchun 130062, China., Hao J; College of Plant Sciences, State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases/Key Laboratory of Zoonosis Research, Ministry of Education, Jilin University, Changchun 130062, China., Ma X; College of Plant Sciences, State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases/Key Laboratory of Zoonosis Research, Ministry of Education, Jilin University, Changchun 130062, China., Jia C; College of Plant Sciences, State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases/Key Laboratory of Zoonosis Research, Ministry of Education, Jilin University, Changchun 130062, China., Liu M; College of Plant Sciences, State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases/Key Laboratory of Zoonosis Research, Ministry of Education, Jilin University, Changchun 130062, China., Wei D; Department of Biology, Institute of Wood Science, University of Hamburg, Hamburg 21031, Germany., Yang S; Zhejiang Provincial Key Laboratory of Chemical Utilization of Forestry Biomass, Zhejiang A&F University, Lin'an, Zhejiang 311300, China., Qin J; College of Plant Sciences, State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases/Key Laboratory of Zoonosis Research, Ministry of Education, Jilin University, Changchun 130062, China.; Shenzhen Research Institute of Jilin University, Shenzhen 518066, China. |
| Abstrakt: |
Botrytis cinerea ( B. cinerea ) and Colletotrichum gloeosporioides ( C. gloeosporioides ) were isolated from the decaying strawberry tissue. The antifungal properties of Monarda didyma essential oil (MEO) and its nanoemulsion were confirmed, demonstrating complete inhibition of the pathogens at concentrations of 0.45 μL/mL (0.37 mg/mL) and 10 μL/mL, respectively. Thymol, a primary component of MEO, was determined as an antimicrobial agent with IC 50 values of 34.51 ( B. cinerea ) and 53.40 ( C. gloeosporioides ) μg/mL. Hippophae rhamnoides oil (HEO) was confirmed as a potent antioxidant, leading to the development of a thymol-HEO-chitosan film designed to act as an antistaling agent. The disease index and weight loss rate can be reduced by 90 and 60%, respectively, with nutrients also being well-preserved, offering an innovative approach to preservative development. Studies on the antifungal mechanism revealed that thymol could bind to FKS1 to disrupt the cell wall, causing the collapse of mitochondrial membrane potential and a burst of reactive oxygen species. |
