Exploring the secrets of marine microorganisms: Unveiling secondary metabolites through metagenomics.

Autor: Wang S; Institute of Marine Science and Technology, Shandong University, Qingdao, Shandong, China.; Qingdao Key Laboratory of Ocean Carbon Sequestration and Negative Emission Technology, Shandong University, Qingdao, China., Li X; Institute of Marine Science and Technology, Shandong University, Qingdao, Shandong, China.; Qingdao Key Laboratory of Ocean Carbon Sequestration and Negative Emission Technology, Shandong University, Qingdao, China., Yang W; School of Computer Science and Technology, Shandong University, Qingdao, Shandong, China., Huang R; Institute of Marine Science and Technology, Shandong University, Qingdao, Shandong, China.; Qingdao Key Laboratory of Ocean Carbon Sequestration and Negative Emission Technology, Shandong University, Qingdao, China.; Global Ocean Negative Carbon Emissions (ONCE) Program Alliance, Qingdao, China.
Jazyk: angličtina
Zdroj: Microbial biotechnology [Microb Biotechnol] 2024 Aug; Vol. 17 (8), pp. e14533.
DOI: 10.1111/1751-7915.14533
Abstrakt: Marine microorganisms are increasingly recognized as primary producers of marine secondary metabolites, drawing growing research interest. Many of these organisms are unculturable, posing challenges for study. Metagenomic techniques enable research on these unculturable microorganisms, identifying various biosynthetic gene clusters (BGCs) related to marine microbial secondary metabolites, thereby unveiling their secrets. This review comprehensively analyses metagenomic methods used in discovering marine microbial secondary metabolites, highlighting tools commonly employed in BGC identification, and discussing the potential and challenges in this field. It emphasizes the key role of metagenomics in unveiling secondary metabolites, particularly in marine sponges and tunicates. The review also explores current limitations in studying these metabolites through metagenomics, noting how long-read sequencing technologies and the evolution of computational biology tools offer more possibilities for BGC discovery. Furthermore, the development of synthetic biology allows experimental validation of computationally identified BGCs, showcasing the vast potential of metagenomics in mining marine microbial secondary metabolites.
(© 2024 The Author(s). Microbial Biotechnology published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje