Transmission of Norwegian reindeer CWD to sheep by intracerebral inoculation results in an unusual phenotype and prion distribution.

Autor: Harpaz E; Department of Production Animal Clinical Sciences, Norwegian University of Life Sciences, Sandnes, Norway., Cazzaniga FA; Unit of Neurology 5 and Neuropathology, Fondazione IRCCS Istituto Neurologico Carlo Besta, Milan, Italy., Tran L; Section for Biohazard and Pathology, Norwegian Veterinary Institute, Ås, Norway., Vuong TT; Section for Biohazard and Pathology, Norwegian Veterinary Institute, Ås, Norway., Bufano G; Unit of Neurology 5 and Neuropathology, Fondazione IRCCS Istituto Neurologico Carlo Besta, Milan, Italy., Salvesen Ø; Department of Production Animal Clinical Sciences, Norwegian University of Life Sciences, Sandnes, Norway.; Åkerblå AS, Haugesund, Norway., Gravdal M; Department of Production Animal Clinical Sciences, Norwegian University of Life Sciences, Sandnes, Norway., Aldaz D; Prion Research Center (PRC) and the Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO, USA., Sun J; Prion Research Center (PRC) and the Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO, USA., Kim S; Prion Research Center (PRC) and the Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO, USA., Celauro L; Department of Neuroscience, Scuola Internazionale Superiore di Studi Avanzati (SISSA), Trieste, Italy., Legname G; Department of Neuroscience, Scuola Internazionale Superiore di Studi Avanzati (SISSA), Trieste, Italy., Telling GC; Prion Research Center (PRC) and the Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO, USA., Tranulis MA; Department of Preclinical Sciences and Pathology, Norwegian University of Life Sciences, Ås, Norway., Benestad SL; Section for Biohazard and Pathology, Norwegian Veterinary Institute, Ås, Norway., Espenes A; Department of Preclinical Sciences and Pathology, Norwegian University of Life Sciences, Ås, Norway., Moda F; Unit of Neurology 5 and Neuropathology, Fondazione IRCCS Istituto Neurologico Carlo Besta, Milan, Italy., Ersdal C; Department of Production Animal Clinical Sciences, Norwegian University of Life Sciences, Sandnes, Norway. cecilie.ersdal@nmbu.no.
Jazyk: angličtina
Zdroj: Veterinary research [Vet Res] 2024 Jul 29; Vol. 55 (1), pp. 94. Date of Electronic Publication: 2024 Jul 29.
DOI: 10.1186/s13567-024-01350-6
Abstrakt: Chronic wasting disease (CWD), a prion disease affecting cervids, has been known in North America (NA) since the 1960s and emerged in Norway in 2016. Surveillance and studies have revealed that there are different forms of CWD in Fennoscandia: contagious CWD in Norwegian reindeer and sporadic CWD in moose and red deer. Experimental studies have demonstrated that NA CWD prions can infect various species, but thus far, there have been no reports of natural transmission to non-cervid species. In vitro and laboratory animal studies of the Norwegian CWD strains suggest that these strains are different from the NA strains. In this work, we describe the intracerebral transmission of reindeer CWD to six scrapie-susceptible sheep. Detection methods included immunohistochemistry (IHC), western blot (WB), enzyme-linked immunosorbent assay (ELISA), real-time quaking-induced conversion (RT-QuIC) and protein misfolding cyclic amplification (PMCA). In the brain, grey matter vacuolation was limited, while all sheep exhibited vacuolation of the white matter. IHC and WB conventional detection techniques failed to detect prions; however, positive seeding activity with the RT-QuIC and PMCA amplification techniques was observed in the central nervous system of all but one sheep. Prions were robustly amplified in the lymph nodes of all animals, mainly by RT-QuIC. Additionally, two lymph nodes were positive by WB, and one was positive by ELISA. These findings suggest that sheep can propagate reindeer CWD prions after intracerebral inoculation, resulting in an unusual disease phenotype and prion distribution with a low amount of detectable prions.
(© 2024. The Author(s).)
Databáze: MEDLINE
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