Aptamer and aptasensor technology for diagnosis of infectious diseases: A mini review.

Autor: Madalgi RK; Molecular Medicine & Microbial Genetics Laboratory, Department of Applied Genetics, Karnatak University, Pavate Nagar, Dharwad, 580003, Karnataka, India. Electronic address: madalgiradhika@gmail.com., Arakera SB; Molecular Medicine & Microbial Genetics Laboratory, Department of Applied Genetics, Karnatak University, Pavate Nagar, Dharwad, 580003, Karnataka, India. Electronic address: drsbarakera@gmail.com., Kulkarni RD; Department of Microbiology SDM College of Medical Sciences and Hospital, Shri Dharmasthala Manjunatheshwara (SDM) University, Dharwad, 580009, Karnataka, India. Electronic address: atul410@yahoo.com.
Jazyk: angličtina
Zdroj: Indian journal of medical microbiology [Indian J Med Microbiol] 2024 Sep-Oct; Vol. 51, pp. 100694. Date of Electronic Publication: 2024 Aug 17.
DOI: 10.1016/j.ijmmb.2024.100694
Abstrakt: Background: Aptamers are not so new a concept, however, it is scarcely discussed by medical fraternity. Aptamers are potent, new identification molecules set to rope in a new technique in the diagnostic arena. Aptamers have started almost a revolution in diagnostic assays since their discovery in the 90s. (Radu S. Current and previous disease outbreaks around the world, U.S. News & World Report. 2020 Mar 13 [cited 2024 Jun 17]. Available from: https://www.usnews.com/news/best-countries/slideshows/20-pandemic-and-epidemic-diseases-according-to-who) provides an overview of pandemics and epidemics as reported by the WHO. It is interesting to note that several endemic and epidemic diseases viz. Chikungunya, Cholera, Crimean-Congo haemorrhagic fever, Ebola virus disease, Hendra virus infection, Influenza, Lassa fever, Marburg virus disease, Meningitis, MERS-CoV (Middle East Respiratory Syndrome Corona Virus), Monkeypox, Nipah virus infection, Novel coronavirus, Plague, Rift Valley fever, SARS (Severe Acute Respiratory Syndrome), Smallpox, Tularaemia, Yellow fever, and Zika virus disease have been identified by the WHO and are being explored for applicability of aptamer technology in their identification.
Objectives: One of the most important necessities to control epidemic or pandemic diseases is early diagnosis. However, the majority of the diagnostic tests for these diseases are available only in tertiary care centres. The objective of this review is to discuss the potential of aptamer technology to provide undemanding, simple, specific, sensitive, and cost-effective diagnostic assays that are useable in remote and field conditions.
Content: Here, we discuss recent advances and approaches in aptamer and aptamer engineering useful in the diagnosis of infectious and non-infectious conditions. This review also discusses a few sensing discoveries which are a gift of advanced engineering and technology using optical and electrochemical aptasensors. It's still a long way to go, and we need to take into account the technological challenges being faced by aptamer-aptasensor technology.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Dr Raghavendra D. Kulkarni reports administrative support and equipment, drugs, or supplies were provided by SDM College of Medical Sciences and Hospital. Dr Raghavendra D. Kulkarni reports a relationship with SDM College of Medical Sciences and Hospital that includes: employment and non-financial support. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Indian Association of Medical Microbiologists. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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