Species-specific bioassays reveal spatial variation in chemical contamination of green sea turtles.

Autor: White-Kiely D; Australian Rivers Institute, Griffith University, Gold Coast, Australia; School of Environment and Science, Griffith University, Gold Coast, Australia. Electronic address: dylan.white-kiely@uwa.edu.au., Finlayson KA; Australian Rivers Institute, Griffith University, Gold Coast, Australia., Limpus CJ; Department of Environment and Science, Queensland, Australia., Johnson M; Australian Rivers Institute, Griffith University, Gold Coast, Australia., van de Merwe JP; Australian Rivers Institute, Griffith University, Gold Coast, Australia; School of Environment and Science, Griffith University, Gold Coast, Australia.
Jazyk: angličtina
Zdroj: Marine environmental research [Mar Environ Res] 2024 Sep; Vol. 200, pp. 106657. Date of Electronic Publication: 2024 Jul 26.
DOI: 10.1016/j.marenvres.2024.106657
Abstrakt: The rapid increase of anthropogenic activity at shipping ports and surrounding coastal areas has been correlated with higher chemical contamination entering the surrounding marine environment. Chemical contaminants in marine environments can lead to significant health problems for green turtles (Chelonia mydas), especially when these contaminants accumulate in their foraging grounds. This study examined the exposure and toxicological effects of chemical contaminants on green turtle cells using a species-specific cell viability assay. Using the QuEChERs extraction, organic contaminants were extracted from 60 blood samples collected from green turtles in three foraging locations: Port Curtis, and two reefs (Heron Reef and Hoskyn-Fairfax Reefs) within the Capricorn Bunker Group of the outer Great Barrier Reef. Blood extracts were tested for cytotoxicity against primary green turtle fibroblast cells using an in vitro resazurin bioassay to assess cell viability. Extracts from Gladstone and Heron Reef indicated significant chemical contamination, at levels high enough to cause adverse health effects of green turtles. Very low toxicity values at the Hoskyn-Fairfax Reefs location indicate its potential to be established as a reference site for the southern Great Barrier Reef.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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