Copper doped hydroxyapatite nanocomposite thin films: synthesis, physico-chemical and biological evaluation.

Autor: Ciobanu CS; National Institute of Materials Physics, Atomistilor Street, No. 405A, P.O. Box MG 07, 077125, Magurele, Romania., Predoi D; National Institute of Materials Physics, Atomistilor Street, No. 405A, P.O. Box MG 07, 077125, Magurele, Romania. dpredoi@gmail.com., Iconaru SL; National Institute of Materials Physics, Atomistilor Street, No. 405A, P.O. Box MG 07, 077125, Magurele, Romania., Predoi MV; Department of Mechanics, University Politehnica of Bucharest, BN 002, 313 Splaiul Independentei, 060042, Bucharest, Romania., Ghegoiu L; National Institute of Materials Physics, Atomistilor Street, No. 405A, P.O. Box MG 07, 077125, Magurele, Romania., Buton N; HORIBA Jobin Yvon S.A.S., 6-18, Rue du Canal, 91165, Longjumeau Cédex, France., Motelica-Heino M; ISTO, UMR 7327 CNRS Université d'Orléans, 1A Rue de la Férollerie, 45071, Orléans CEDEX 2, France.
Jazyk: angličtina
Zdroj: Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine [Biometals] 2024 Jul 29. Date of Electronic Publication: 2024 Jul 29.
DOI: 10.1007/s10534-024-00620-2
Abstrakt: Cu-doped hydroxyapatite (CuHAp) thin films were obtained using spin coating method. To make these thin films, CuHAp suspensions obtained by sol-gel method were used. The coatings obtained were thermally treated at 500 °C. After the thermal treatment, the thin films were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM). Moreover, the stability of the suspensions before being used to obtain the thin films was certified by dynamic light scattering (DLS), zeta potential methods and ultrasound measurements. In the XRD patterns, the peaks associated with hexagonal hydroxyapatite were identified in accordance with JCPDS no. 09-0432. EDS and XPS results confirmed the presence of Cu ions in the samples. Data about the morphological features and chemical composition of CuHAp thin films were obtained by performing scanning electron microscopy (SEM) measurements. Our results suggest that the CuHAp thin films surface is continuous and homogenous. The presence of the functional groups in the CuHAp thin films was confirmed by Fourier-transform infrared spectroscopy (FTIR) and Raman spectroscopy studies. Information about the surface topography of the CuHAp thin films has been obtained using atomic force microscopy (AFM). The AFM images determined that the surface topography of the CuHAp thin layer is homogenous and continuous without presenting any unevenness or fissures. The cytotoxicity of CuHAp thin films was assessed using human gingival fibroblasts (HGF-1) cells. The results of the cell viability assays demonstrated that the thin films presented good biocompatible properties towards the HGF-1 cells. Additionally, the adherence and development of HGF-1 cells on the surface of CuHAp thin films were determined using AFM. The AFM surface topographies highlighted that the CuHAp thin film's surface favored the attachment and proliferation of HGF-1 cells on their surface.
(© 2024. The Author(s).)
Databáze: MEDLINE
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