Effect of curcumin-donepezil combination on spatial memory, astrocyte activation, and cholinesterase expressions in brain of scopolamine-treated rats.

Autor: Ogunsuyi OB; Department of Biomedical Technology, School of Basic Medical Sciences, The Federal University of Technology, Akure, Nigeria. opeyemiogunsuyi@gmail.com.; Drosophila Research Lab, Functional Foods and Nutraceuticals Unit, The Federal University of Technology, Akure, Nigeria. opeyemiogunsuyi@gmail.com., Ogunruku OO; Department of Biochemistry and Molecular Biology, Obafemi Awolowo University, Ile-Ife, Nigeria. omodesola.oluwafisayo@gmail.com., Umar HI; Department of Biochemistry, The Federal University of Technology, Akure, Nigeria.; Molecular Biology and Bioinformatics Lab, Department of Biochemistry, The Federal University of Technology, Akure, Nigeria., Oboh G; Drosophila Research Lab, Functional Foods and Nutraceuticals Unit, The Federal University of Technology, Akure, Nigeria.; Department of Biochemistry, The Federal University of Technology, Akure, Nigeria.
Jazyk: angličtina
Zdroj: Molecular biology reports [Mol Biol Rep] 2024 Jul 29; Vol. 51 (1), pp. 864. Date of Electronic Publication: 2024 Jul 29.
DOI: 10.1007/s11033-024-09712-1
Abstrakt: Background: The study investigated the effect of co-administration of curcumin and donepezil on several markers of cognitive function (such as spatial memory, astrocyte activation, cholinesterase expressions) in the brain cortex and hippocampus of scopolamine-treated rats.
Method and Results: For seven consecutive days, a pre-treatment of curcumin (50 mg/kg) and/or donepezil (2.5 mg/kg) was administered. On the seventh day, scopolamine (1 mg/kg) was administered to elicit cognitive impairment, 30 min before memory test was conducted. This was followed by evaluating changes in spatial memory, cholinesterase, and adenosine deaminase (ADA) activities, as well as nitric oxide (NO) level were determined. Additionally, RT-qPCR for glial fibrillary acidic protein (GFAP) and cholinesterase gene expressions was performed in the brain cortex and hippocampus. Also, GFAP immunohistochemistry  of the brain tissues for neuronal injury were performed in the brain cortex and hippocampus. In comparison to the control group, rats given scopolamine had impaired memory, higher levels of acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and ADA activities, as well as elevated markers of oxidative stress. In addition to enhanced GFAP immunoreactivity, there was also overexpression of the GFAP and BChE genes in the brain tissues. The combination of curcumin and donepezil was, however, observed to better ameliorate these impairments in comparison to the donepezil-administered rat group.
Conclusion: Hence, this evidence provides more mechanisms to support the hypothesis that the concurrent administration of curcumin and donepezil mitigates markers of cognitive dysfunction in scopolamine-treated rat model.
(© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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