p38-MAPK is prerequisite for the synthesis of SARS-CoV-2 protein.

Autor: Mittal P; National Centre for Veterinary Type Cultures, ICAR-National Research Centre on Equines, Hisar, Haryana 125001 India.; Om Sterling Global University (OSGU), Hisar, Haryana 125001 India., Khandelwal N; National Centre for Veterinary Type Cultures, ICAR-National Research Centre on Equines, Hisar, Haryana 125001 India., Chander Y; National Centre for Veterinary Type Cultures, ICAR-National Research Centre on Equines, Hisar, Haryana 125001 India., Verma A; National Centre for Veterinary Type Cultures, ICAR-National Research Centre on Equines, Hisar, Haryana 125001 India., Kumar R; National Centre for Veterinary Type Cultures, ICAR-National Research Centre on Equines, Hisar, Haryana 125001 India., Putatunda C; Om Sterling Global University (OSGU), Hisar, Haryana 125001 India., Barua S; National Centre for Veterinary Type Cultures, ICAR-National Research Centre on Equines, Hisar, Haryana 125001 India., Gulati BR; National Centre for Veterinary Type Cultures, ICAR-National Research Centre on Equines, Hisar, Haryana 125001 India., Kumar N; National Centre for Veterinary Type Cultures, ICAR-National Research Centre on Equines, Hisar, Haryana 125001 India.
Jazyk: angličtina
Zdroj: Virusdisease [Virusdisease] 2024 Jun; Vol. 35 (2), pp. 329-337. Date of Electronic Publication: 2024 May 27.
DOI: 10.1007/s13337-024-00873-y
Abstrakt: The inhibition of p38 mitogen-activated protein kinase (p38-MAPK) by small molecule chemical inhibitors was previously shown to impair severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) replication, however, mechanisms underlying antiviral activity remains unexplored. In this study, reduced growth of SARS-CoV-2 in p38- α knockout Vero cells, together with enhanced viral yield in cells transfected with construct expressing p38 α , suggested that p38-MAPK is essential for the propagation of SARS-CoV-2. The SARS-CoV-2 was also shown to induce phosphorylation (activation) of p38, at time when transcription/translational activities are considered to be at the peak levels. Further, we demonstrated that p38 supports viral RNA/protein synthesis without affecting viral attachment, entry, and budding in the target cells. In conclusion, we provide mechanistic insights on the regulation of SARS-CoV-2 replication by p38 MAPK.
Competing Interests: Conflict of interestThe authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(© The Author(s), under exclusive licence to Indian Virological Society 2024.)
Databáze: MEDLINE
Externí odkaz: