Topical blood products modulate the effects of ophthalmic antibiotics against common bacterial pathogens in dogs with infectious keratitis.
| Autor: | Kubai MA; Department of Veterinary Clinical Sciences, Iowa State University, College of Veterinary Medicine, Ames, IA, United States., Roy MM; Department of Veterinary Clinical Sciences, Iowa State University, College of Veterinary Medicine, Ames, IA, United States., Stinman CC; Veterinary Diagnostic Laboratory, Iowa State University, College of Veterinary Medicine, Ames, IA, United States., Kenne DE; Veterinary Diagnostic Laboratory, Iowa State University, College of Veterinary Medicine, Ames, IA, United States., Allbaugh RA; Department of Veterinary Clinical Sciences, Iowa State University, College of Veterinary Medicine, Ames, IA, United States., Sebbag L; Department of Veterinary Clinical Sciences, Iowa State University, College of Veterinary Medicine, Ames, IA, United States.; Koret School of Veterinary Medicine, Hebrew University of Jerusalem, Rehovot, Israel. |
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| Jazyk: | angličtina |
| Zdroj: | Frontiers in veterinary science [Front Vet Sci] 2024 Jul 12; Vol. 11, pp. 1417842. Date of Electronic Publication: 2024 Jul 12 (Print Publication: 2024). |
| DOI: | 10.3389/fvets.2024.1417842 |
| Abstrakt: | Bacterial keratitis is a common and serious condition that often leads to vision impairment and potential loss of the eye if not treated promptly and adequately. Topical blood products are often used concurrently with topical antibiotics, helping to mitigate corneal 'melt' from proteases released on the ocular surface. However, blood products are rich in albumin and could affect the efficacy of antibiotics due to drug-protein binding. In this study, serum and plasma samples were harvested from 10 healthy dogs and 10 healthy horses, obtaining fresh and frozen (1 month at -20°C) aliquots for in vitro experiments. Albumin levels were quantified using species-specific ELISA kits. Thirty bacteria (10 Staphylococcus pseudintermedius , 10 Streptococcus canis , 10 Pseudomonas aeruginosa ), isolated from canine patients with infectious keratitis, were each tested with blank plates as well as commercial susceptibility plates (Sensititre™ JOEYE2) to assess the minimal inhibitory concentration (MIC) of 17 different antibiotics in the absence (control) or presence of eight test groups: serum or plasma (fresh or frozen) from canines or equines. Albumin concentrations ranged from 13.8-14.6 mg/mL and 25.9-26.5 mg/mL in canine and equine blood products, respectively. A direct antimicrobial effect was observed mostly with equine vs. canine blood products (specifically serum and to a lesser degree plasma), and mostly for Staphylococcus pseudintermedius isolates. MICs generally increased in the presence of blood products (up to 10.8-fold), although MICs also decreased (down to 0.25-fold) for selected antibiotics and ocular pathogens. Median (range) fold changes in MICs were significantly greater ( p = 0.004) with the canine blood products [2 (0.67-8.1)] than the equine blood products [2 (0.5-5)]. In practice, clinicians should consider equine over canine blood products (lesser impact on antimicrobial susceptibility), serum over plasma (greater antimicrobial effects), and administering the blood product ≥15 min following the last antibiotic eyedrop to minimize the amount of albumin-antibiotic binding in tear film. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Kubai, Roy, Stinman, Kenne, Allbaugh and Sebbag.) |
| Databáze: | MEDLINE |
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