Nanocomposite system with photoactive phloxine B eradicates resistant Staphylococcus aureus .

Autor:	Bilská K; Comenius University in Bratislava, Faculty of Natural Sciences, Department of Microbiology and Virology, Ilkovičova 6, 84215, Bratislava, Slovak Republic., Bujdák J; Comenius University in Bratislava, Faculty of Natural Sciences, Department of Physical and Theoretical Chemistry, Ilkovičova 6, 84215, Bratislava, Slovak Republic.; Institute of Inorganic Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 84536, Bratislava, Slovak Republic., Bujdáková H; Comenius University in Bratislava, Faculty of Natural Sciences, Department of Microbiology and Virology, Ilkovičova 6, 84215, Bratislava, Slovak Republic.
Jazyk:	angličtina
Zdroj:	Heliyon [Heliyon] 2024 Jun 26; Vol. 10 (13), pp. e33660. Date of Electronic Publication: 2024 Jun 26 (Print Publication: 2024).
DOI:	10.1016/j.heliyon.2024.e33660
Abstrakt:	Nanomaterials modified with hybrid films functionalized with photoactive compounds can be an effective system to prevent and eradicate biofilms on medical devices. The aim of this research was to extend current knowledge on nanomaterial comprised of polyurethane (PU) modified with a nanocomposite film of organoclay with the functionalized photosensitizer (PS) phloxine B (PhB). Particles of the clay mineral saponite were, at first modified by octadecyltrimethylammonium cations to activate the surface for PhB adsorption. The colloids were filtered to get silicate films on polytetrafluoroethylene membrane filters, which were layered with a liquid mixture of PU precursors. The penetration of PU into the silicate formed a thin nanocomposite film. This nanomaterial demonstrated excellent effectiveness against methicillin-resistant S. aureus (MRSA) resistant to fluoroquinolones (L12 and S61, respectively). It showed more than 1000- and 10 000-fold inhibition of biofilm growth after irradiation with green laser compared to the unmodified PU material. Principal component analysis and multiple linear regression showed that the effectiveness of the nanomaterial was not influenced by virulence factors such as the expression of efflux pumps of the Nor family, the adhesin PIA encoded by the icaADBC operon or the robustness of the biofilms. However, the presence of organoclay, PhB and irradiation had a significant effect on the anti -biofilm properties of the nanocomposite. The anti-microbial properties of the material were strengthened after irradiation, because of high reactive oxygen species release (more than 14-fold compared to non-irradiated sample). Materials based on photoactive molecules can represent a worthwhile pathway towards the development of more complex nanomaterials applicable in various fields of medicine. Competing Interests: Authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2024 The Authors.)
Databáze:	MEDLINE
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