| Autor: |
Aguilar GR; Department of Biological Sciences, Howard Hughes Medical Institute, Columbia University, New York, NY., Vidal B; Department of Biological Sciences, Howard Hughes Medical Institute, Columbia University, New York, NY., Ji H; Department of Biological Sciences, Howard Hughes Medical Institute, Columbia University, New York, NY., Evenblij J; Department of Biological Sciences, Howard Hughes Medical Institute, Columbia University, New York, NY.; Technische Universität, Braunschweig, Germany., Ji H; Department of Biomedical Engineering, Ohio State University, Columbus, OH., Valperga G; Department of Biological Sciences, Howard Hughes Medical Institute, Columbia University, New York, NY., Liao CP; Department of Biological Sciences, Howard Hughes Medical Institute, Columbia University, New York, NY., Fang-Yen C; Department of Biomedical Engineering, Ohio State University, Columbus, OH., Hobert O; Department of Biological Sciences, Howard Hughes Medical Institute, Columbia University, New York, NY. |
| Abstrakt: |
Throughout the animal kingdom, several members of the basic helix-loop-helix (bHLH) family act as proneural genes during early steps of nervous system development. Roles of bHLH genes in specifying terminal differentiation of postmitotic neurons have been less extensively studied. We analyze here the function of five C. elegans bHLH genes, falling into three phylogenetically conserved subfamilies, which are continuously expressed in a very small number of postmitotic neurons in the central nervous system. We show (a) that two orthologs of the vertebrate bHLHb4/b5 genes, called hlh-17 and hlh-32 , function redundantly to specify the identity of a single head interneuron (AUA), as well as an individual motor neuron (VB2), (b) that the PTF1a ortholog hlh-13 acts as a terminal selector to control terminal differentiation and function of the sole octopaminergic neuron class in C. elegans , RIC, and (c) that the NHLH1/2 ortholog hlh-15 controls terminal differentiation and function of the peptidergic AVK head interneuron class, a known neuropeptidergic signaling hub in the animal. Strikingly, through null mutant analysis and cell-specific rescue experiments, we find that loss of hlh-15/NHLH in the peptidergic AVK neurons and the resulting abrogation of neuropeptide secretion causes a substantially expanded lifespan of the animal, revealing an unanticipated impact of a central, peptidergic hub neuron in regulating lifespan, which we propose to be akin to hypothalamic control of lifespan in vertebrates. Taken together, our functional analysis reveals themes of bHLH gene function during terminal differentiation that are complementary to the earlier lineage specification roles of other bHLH family members. However, such late functions are much more sparsely employed by members of the bHLH transcription factor family, compared to the function of the much more broadly employed homeodomain transcription factor family. |
