An innovative dual-organelle targeting NIR fluorescence probe for detecting hydroxyl radicals in biosystem and inflammation models.

Autor: Ma J; College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang, Henan 455000, China; Department of Chemistry, Clemson University, Clemson 29634, SC, United States. Electronic address: junyanma@aynu.edu.cn., Zhao M; College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang, Henan 455000, China., Kong X; College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang, Henan 455000, China., Xie H; State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China., Li H; College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang, Henan 455000, China., Jiao Z; College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang, Henan 455000, China., Zhang Z; College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang, Henan 455000, China; Department of Energy and Resources Engineering, College of Engineering, Peking University, Beijing 100871, China. Electronic address: zzx1982@pku.edu.cn.
Jazyk: angličtina
Zdroj: Bioorganic chemistry [Bioorg Chem] 2024 Oct; Vol. 151, pp. 107678. Date of Electronic Publication: 2024 Jul 26.
DOI: 10.1016/j.bioorg.2024.107678
Abstrakt: The hydroxyl radical (OH) is highly reactive and plays a significant role in a number of physiological and pathological processes within biosystems. Aberrant changes in the level of hydroxyl radical are associated with many disorders including tumor, inflammatory and cardiovascular diseases. Thus, detecting reactive oxygen species (ROS) in biological systems and imaging them is highly significant. In this work, a novel fluorescent probe (HR-DL) has been developed, targeting two organelles simultaneously. The probe is based on a coumarin-quinoline structure and exhibits high selectivity and sensitivity towards hydroxyl radicals (OH). When reacting with OH, the hydrogen abstraction process released its long-range π-conjugation and ICT processes, leading to a substantial red-shift in wavelength. This probe has the benefits of good water solubility (in its oxidative state), short response time (within 10 s), and unique dual lysosome/mitochondria targeting capabilities. It has been applied for sensing OH in biosystem and inflammation mice models.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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