Protocol for continuous intravenous drug delivery with implantable iPrecio pump in free-moving rats and mice.
| Autor: | Giri T; Department of Anesthesiology, Washington University School of Medicine, St. Louis, MO 63110, USA. Electronic address: tusargiri@wustl.edu., Palanisamy A; Department of Anesthesiology, Washington University School of Medicine, St. Louis, MO 63110, USA. Electronic address: arvind.palanisamy@wustl.edu. |
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| Jazyk: | angličtina |
| Zdroj: | STAR protocols [STAR Protoc] 2024 Sep 20; Vol. 5 (3), pp. 103224. Date of Electronic Publication: 2024 Jul 27. |
| DOI: | 10.1016/j.xpro.2024.103224 |
| Abstrakt: | Variable-rate delivery of intravenous drugs is difficult to achieve with a tethered infusion system in a freely moving animal. Here, we present a protocol for continuous intravenous delivery of oxytocin in pregnant rats and mice. We describe steps for using an implantable, preprogrammed, microprocessor-controlled infusion pump connected to the jugular vein to induce labor. This protocol can be adapted to a variety of experimental paradigms in non-pregnant animals where precise intravenous pharmacological manipulation is desired. For complete details on the use and execution of this protocol, please refer to Giri et al. 1 , 2 . Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.) |
| Databáze: | MEDLINE |
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