Sensitivity-improved SERS detection of SARS-CoV-2 spike protein by Au NPs/COFs integrated with catalytic-hairpin-assembly amplification technology.

Autor: Huang Q; School of Science, Xihua University, Chengdu, Sichuan, 610039, China., Zhou N; School of Science, Xihua University, Chengdu, Sichuan, 610039, China; Food Microbiology, Key Laboratory of Sichuan Province, Xihua University, Chengdu, 610039, Sichuan, China., Peng J; School of Science, Xihua University, Chengdu, Sichuan, 610039, China., Zeng X; School of Science, Xihua University, Chengdu, Sichuan, 610039, China., Du L; School of Science, Xihua University, Chengdu, Sichuan, 610039, China., Zhao Y; School of Science, Xihua University, Chengdu, Sichuan, 610039, China; Asymmetric Synthesis and Chiral Technology Key Laboratory of Sichuan Province, Chengdu, 610039, Sichuan, China. Electronic address: zhao518yan@163.com., Luo X; School of Science, Xihua University, Chengdu, Sichuan, 610039, China; Asymmetric Synthesis and Chiral Technology Key Laboratory of Sichuan Province, Chengdu, 610039, Sichuan, China. Electronic address: xajunluo@hotmail.com.
Jazyk: angličtina
Zdroj: Analytica chimica acta [Anal Chim Acta] 2024 Aug 22; Vol. 1318, pp. 342924. Date of Electronic Publication: 2024 Jun 29.
DOI: 10.1016/j.aca.2024.342924
Abstrakt: Background: The COVID-19 pandemic, caused by the novel coronavirus, has had a profound impact on global health and economies worldwide. This unprecedented crisis has affected individuals, communities, and nations in diverse manners. Developing simple and accurate diagnostic methods is an imperative task for frequent testing to mitigate the spread of the virus. Among these methods, SARS-CoV-2 antigen tests in clinical specimens have emerged as a promising diagnostic method for COVID-19 due to their sensitive and accurate detection of spike (S) protein, which plays a crucial role in viral infection initiation.
Results: In this work, a dual-signal amplification surface enhanced Raman scattering (SERS)-based S protein biosensor was constructed based on Au NPs/COFs and enzyme-free catalytic hairpin assembly (CHA) amplification method. The approach relies on a released free DNA sequence (T), which is generated from the competition reaction between Aptamer/T and Aptamer/S protein, to trigger a CHA reaction. Due to the high binding affinity and selectivity between the S protein and its aptamer, CHA process was triggered with the maximum SERS tags (H2-conjugated Au@4-mercaptobenzonitrile@Ag) anchored onto Au NPs/COFs substrate surface. This SERS platform could detect the S protein at concentrations with high sensitivity (limit of detection = 3.0 × 10 -16  g/mL), wide detection range (1 × 10 -16 to 1 × 10 -11  g/mL), acceptable reproducibility (relative standard deviation = 7.01 %) and excellent specificity. The biosensor was also employed to detect S protein in artificial human salivas.
Significance: Thus, this study not only developed a novel Au NPs/COFs substrate exhibiting strong SERS enhancement ability and high reproducibility, but also proposed a promising dual-signal amplification SERS-based diagnostic method for COVID-19, holding immense potential for the detection of a wide range of antigens and infectious diseases in future applications.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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