Polyaniline-based bovine serum albumin imprinted electrochemical sensor for ultra-trace-level detection in clinical and food safety applications.

Autor: Ting WT; Department of Electrical and Computer Engineering, McMaster University, 1280 Main Street West, Hamilton, ON L8S 4K1, Canada; Department of Chemical Engineering, National Taiwan University of Science and Technology, No. 43 Keelung Road Section 4, Taipei 106, Taiwan; Taiwan Building Technology Center, National Taiwan University of Science and Technology, No. 43 Keelung Road Section 4, Taipei 106, Taiwan., Ali MY; Department of Electrical and Computer Engineering, McMaster University, 1280 Main Street West, Hamilton, ON L8S 4K1, Canada., Mitea V; Department of Electrical and Computer Engineering, McMaster University, 1280 Main Street West, Hamilton, ON L8S 4K1, Canada; School of Biomedical Engineering, McMaster University, 1280 Main Street West, Hamilton, ON L8S 4K1, Canada., Wang MJ; Department of Chemical Engineering, National Taiwan University of Science and Technology, No. 43 Keelung Road Section 4, Taipei 106, Taiwan. Electronic address: mjwang@mail.ntust.edu.tw., Howlader MMR; Department of Electrical and Computer Engineering, McMaster University, 1280 Main Street West, Hamilton, ON L8S 4K1, Canada; School of Biomedical Engineering, McMaster University, 1280 Main Street West, Hamilton, ON L8S 4K1, Canada. Electronic address: howladm@mcmaster.ca.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 Oct; Vol. 277 (Pt 2), pp. 134137. Date of Electronic Publication: 2024 Jul 25.
DOI: 10.1016/j.ijbiomac.2024.134137
Abstrakt: Monitoring bovine serum albumin (BSA) at ultra-low levels is crucial for clinical and food safety applications, as it plays a significant role in identifying various health conditions and potential risks, necessitating fast, trace-level detection of BSA. This study proposes an approach to address these challenges by employing molecularly imprinted polymer (MIP) to develop an ultra-trace-level and cost-effective BSA sensing platform. The MIP electrochemical sensor was developed using polyaniline (PANI) combined with the protein crosslinker glutaraldehyde (GA) to optimize BSA surface imprinting in the MIP. As a result, the sensor achieves a sensitivity of 1.24 μA/log(pg/mL), with a picomolar detectable limit of 2.3 pg/mL (0.035 pM) and a wide detection range from 20 pg/mL to 200,000 pg/mL (0.303 pM to 3030 pM), making it suitable for clinical and food safety applications. Additionally, the study explores the interaction between an acidic surfactant protein eluent (acetic acid with sodium dodecyl sulfate, AcOH-SDS) and BSA vacant sites, enhancing recognition and re-binding. The PANI-based MIP sensor demonstrates initial feasibility and practicality in commercial milk and real human serum, opening avenues for early disease detection and ensuring food safety in BSA-related immune responses.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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