Plastic-degrading microbial communities reveal novel microorganisms, pathways, and biocatalysts for polymer degradation and bioplastic production.

Autor: Roman EKB; Department of Biochemistry and Tissue Biology, Institute of Biology, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil., Ramos MA; Laboratory of Molecular Sciences, University of Sorocaba (UNISO), Sorocaba, SP, Brazil; Programa de Processos Tecnológicos e Ambientais, University of Sorocaba (UNISO), Sorocaba, SP, Brazil., Tomazetto G; Department of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, PA, United States., Foltran BB; Laboratory of Molecular Sciences, University of Sorocaba (UNISO), Sorocaba, SP, Brazil., Galvão MH; Laboratory of Molecular Sciences, University of Sorocaba (UNISO), Sorocaba, SP, Brazil., Ciancaglini I; Department of Biochemistry and Tissue Biology, Institute of Biology, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil; Laboratory of Molecular Sciences, University of Sorocaba (UNISO), Sorocaba, SP, Brazil., Tramontina R; Department of Biochemistry and Tissue Biology, Institute of Biology, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil; Laboratory of Molecular Sciences, University of Sorocaba (UNISO), Sorocaba, SP, Brazil., de Almeida Rodrigues F; Laboratory of Molecular Sciences, University of Sorocaba (UNISO), Sorocaba, SP, Brazil., da Silva LS; Laboratory of Molecular Sciences, University of Sorocaba (UNISO), Sorocaba, SP, Brazil., Sandano ALH; Laboratory of Molecular Sciences, University of Sorocaba (UNISO), Sorocaba, SP, Brazil., Fernandes DGDS; Centro de Ciências Naturais e Humanas (CCNH), Universidade Federal do ABC (UFABC), Santo André, SP, Brazil., Almeida DV; Centro de Ciências Naturais e Humanas (CCNH), Universidade Federal do ABC (UFABC), Santo André, SP, Brazil., Baldo DA; Laboratory of Applied Nuclear Physics, University of Sorocaba (UNISO), Sorocaba, SP, Brazil., de Oliveira Junior JM; Laboratory of Applied Nuclear Physics, University of Sorocaba (UNISO), Sorocaba, SP, Brazil., Garcia W; Centro de Ciências Naturais e Humanas (CCNH), Universidade Federal do ABC (UFABC), Santo André, SP, Brazil., Damasio A; Department of Biochemistry and Tissue Biology, Institute of Biology, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil., Squina FM; Laboratory of Molecular Sciences, University of Sorocaba (UNISO), Sorocaba, SP, Brazil; Programa de Processos Tecnológicos e Ambientais, University of Sorocaba (UNISO), Sorocaba, SP, Brazil. Electronic address: fabio.squina@prof.uniso.br.
Jazyk: angličtina
Zdroj: The Science of the total environment [Sci Total Environ] 2024 Nov 01; Vol. 949, pp. 174876. Date of Electronic Publication: 2024 Jul 25.
DOI: 10.1016/j.scitotenv.2024.174876
Abstrakt: Plastics derived from fossil fuels are used ubiquitously owing to their exceptional physicochemical characteristics. However, the extensive and short-term use of plastics has caused environmental challenges. The biotechnological plastic conversion can help address the challenges related to plastic pollution, offering sustainable alternatives that can operate using bioeconomic concepts and promote socioeconomic benefits. In this context, using soil from a plastic-contaminated landfill, two consortia were established (ConsPlastic-A and -B) displaying versatility in developing and consuming polyethylene or polyethylene terephthalate as the carbon source of nutrition. The ConsPlastic-A and -B metagenomic sequencing, taxonomic profiling, and the reconstruction of 79 draft bacterial genomes significantly expanded the knowledge of plastic-degrading microorganisms and enzymes, disclosing novel taxonomic groups associated with polymer degradation. The microbial consortium was utilized to obtain a novel Pseudomonas putida strain (BR4), presenting a striking metabolic arsenal for aromatic compound degradation and assimilation, confirmed by genomic analyses. The BR4 displays the inherent capacity to degrade polyethylene terephthalate (PET) and produce polyhydroxybutyrate (PHB) containing hydroxyvalerate (HV) units that contribute to enhanced copolymer properties, such as increased flexibility and resistance to breakage, compared with pure PHB. Therefore, BR4 is a promising strain for developing a bioconsolidated plastic depolymerization and upcycling process. Collectively, our study provides insights that may extend beyond the artificial ecosystems established during our experiments and supports future strategies for effectively decomposing and valorizing plastic waste. Furthermore, the functional genomic analysis described herein serves as a valuable guide for elucidating the genetic potential of microbial communities and microorganisms in plastic deconstruction and upcycling.
Competing Interests: Declaration of competing interest The authors declare that they have no competing financial interests or personal relationships that could have appeared to influence this study.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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