Screening and identification of peptidyl arginine deiminase 4 inhibitors from herbal plants extracts and purified natural products by a trypsin assisted sensitive immunoassay based on streptavidin magnetic beads.

Autor: Zhao J; Key Laboratory of Resource Biology and Modern Biotechnology in Western China, College of Life Science, Northwest University, Xi'an, Shaanxi Province, 710069, PR China., Zhang S; Key Laboratory of Resource Biology and Modern Biotechnology in Western China, College of Life Science, Northwest University, Xi'an, Shaanxi Province, 710069, PR China., Dong J; Key Laboratory of Resource Biology and Modern Biotechnology in Western China, College of Life Science, Northwest University, Xi'an, Shaanxi Province, 710069, PR China., Chen X; Key Laboratory of Resource Biology and Modern Biotechnology in Western China, College of Life Science, Northwest University, Xi'an, Shaanxi Province, 710069, PR China., Zuo H; Key Laboratory of Resource Biology and Modern Biotechnology in Western China, College of Life Science, Northwest University, Xi'an, Shaanxi Province, 710069, PR China., Li Y; Key Laboratory of Resource Biology and Modern Biotechnology in Western China, College of Life Science, Northwest University, Xi'an, Shaanxi Province, 710069, PR China., Gao C; Key Laboratory of Resource Biology and Modern Biotechnology in Western China, College of Life Science, Northwest University, Xi'an, Shaanxi Province, 710069, PR China., Zhao Z; Key Laboratory of Resource Biology and Modern Biotechnology in Western China, College of Life Science, Northwest University, Xi'an, Shaanxi Province, 710069, PR China., Qiu X; Xiamen Jinnuohua Biotechnology Co., Ltd., Xiamen, Fujian, 361000, PR China., Tang Z; Xiamen Jinnuohua Biotechnology Co., Ltd., Xiamen, Fujian, 361000, PR China., Deng N; Instrumental Analysis Center, Xi'an Jiaotong University, Xi'an, Shaanxi Province, 710049, PR China., Zhao W; School of Pharmacy, Shenzhen Technology University, Shenzhen, Guangdong, 518118, PR China. Electronic address: zhaoweining@sztu.edu.cn., Ou J; Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry & Materials Science, Northwest University, Xi'an, Shaanxi Province, 710069, PR China. Electronic address: junjieou@nwu.edu.cn., Bian Y; Key Laboratory of Resource Biology and Modern Biotechnology in Western China, College of Life Science, Northwest University, Xi'an, Shaanxi Province, 710069, PR China. Electronic address: bianyy@nwu.edu.cn.
Jazyk: angličtina
Zdroj: Talanta [Talanta] 2024 Nov 01; Vol. 279, pp. 126611. Date of Electronic Publication: 2024 Jul 24.
DOI: 10.1016/j.talanta.2024.126611
Abstrakt: Peptidyl arginine deiminase 4 (PAD4) plays a critical role in many autoimmune diseases including rheumatoid arthritis. Herein, a trypsin assisted highly immunoassay method was established to determine PAD4 activity and screen potent inhibitors from herbal plants extracts and purified natural products. The method was applied to determine endogenous PAD4 activity in both cell and tissue lysates, as well as the inhibitory effects of 20 herbal plants and 50 purified natural products. The Cinnamomi ramulus extract showed strongest inhibitory potency with IC 50 value lower than 5 μg/mL. Meanwhile, pyrroloquinoline quinone (PQQ), widely used as a dietary supplement, was discovered as a promising PAD4 inhibitor with an IC 50 value lower than 4 μM. The inhibition kinetic analysis, drug affinity response target stability (DARTS) and molecular docking were performed to confirm the interaction between PQQ and PAD4. This method has great potential for researchers to monitor activities and discover potential inhibitors of PAD4.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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