Electrochemical detection of poly(3-hydroxybutyrate) production from Burkholderia glumae MA13 using a molecularly imprinted polymer-reduced graphene oxide modified electrode.

Autor: da Conceição E; Instituto de Química, Universidade Estadual Paulista (UNESP), Araraquara, 14800-060, Brazil.; Instituto de Pesquisa em Bioenergia (IPBEN), Universidade Estadual Paulista (UNESP), Araraquara, 14800-060, Brazil., Buffon E; Instituto de Química, Universidade Estadual Paulista (UNESP), Araraquara, 14800-060, Brazil.; Instituto de Pesquisa em Bioenergia (IPBEN), Universidade Estadual Paulista (UNESP), Araraquara, 14800-060, Brazil., Beluomini MA; Instituto de Pesquisa em Bioenergia (IPBEN), Universidade Estadual Paulista (UNESP), Araraquara, 14800-060, Brazil. maisa.beluomini@unesp.br.; Departamento de Biotecnologia, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista (UNESP), Jaboticabal, Brasil. maisa.beluomini@unesp.br., Falone MF; Instituto de Química, Universidade Estadual Paulista (UNESP), Araraquara, 14800-060, Brazil.; Instituto de Pesquisa em Bioenergia (IPBEN), Universidade Estadual Paulista (UNESP), Araraquara, 14800-060, Brazil., de Andrade FB; Instituto de Biociências, Universidade Estadual Paulista (UNESP), Rio Claro, 13506-900, Brazil.; Instituto de Pesquisa em Bioenergia (IPBEN), Universidade Estadual Paulista (UNESP), Rio Claro, 13500-230, Brazil., Contiero J; Instituto de Biociências, Universidade Estadual Paulista (UNESP), Rio Claro, 13506-900, Brazil.; Instituto de Pesquisa em Bioenergia (IPBEN), Universidade Estadual Paulista (UNESP), Rio Claro, 13500-230, Brazil., Stradiotto NR; Instituto de Química, Universidade Estadual Paulista (UNESP), Araraquara, 14800-060, Brazil.; Instituto de Pesquisa em Bioenergia (IPBEN), Universidade Estadual Paulista (UNESP), Araraquara, 14800-060, Brazil.
Jazyk: angličtina
Zdroj: Mikrochimica acta [Mikrochim Acta] 2024 Jul 27; Vol. 191 (8), pp. 492. Date of Electronic Publication: 2024 Jul 27.
DOI: 10.1007/s00604-024-06580-1
Abstrakt: The development and application of an electrochemical sensor is reported for detection of poly(3-hydroxybutyrate) (P3HB) - a bioplastic derived from agro-industrial residues. To overcome the challenges of molecular imprinting of macromolecules such as P3HB, this study employed methanolysis reaction to break down the P3HB biopolymer chains into methyl 3-hydroxybutyrate (M3HB) monomers. Thereafter, M3HB were employed as the target molecules in the construction of molecularly imprinted sensors. The electrochemical device was then prepared by electropolymerizing a molecularly imprinted poly (indole-3-acetic acid) thin film on a glassy carbon electrode surface modified with reduced graphene oxide (GCE/rGO-MIP) in the presence of M3HB. Electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), scanning electron microscopy with field emission gun (SEM-FEG), Raman spectroscopy, attenuated total reflection Fourier-transform infrared (ATR-FTIR) and X-ray Photoelectron Spectroscopy (XPS) were employed to characterize the electrode surface. Under ideal conditions, the MIP sensor exhibited a wide linear working range of 0.1 - 10 nM and a detection limit of 0.3 pM (n = 3). The sensor showed good repeatability, selectivity, and stability over time. For the sensor application, the bioproduction of P3HB was carried out in a bioreactor containing the Burkholderia glumae MA13 strain and sugarcane byproducts as a supplementary carbon source. The analyses were validated through recovery assays, yielding recovery values between 102 and 104%. These results indicate that this MIP sensor can present advantages in the monitoring of P3HB during the bioconversion process.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)
Databáze: MEDLINE
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